Abstract
Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich–protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
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Acknowledgements
We thank D.A. Kedziorek, V.P. Chacko and J. Zhang for experimental assistance. Supported by National Institutes of Health grants CA103175, NS045062, EB005252 and K01 EB006394.
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Gilad, A., McMahon, M., Walczak, P. et al. Artificial reporter gene providing MRI contrast based on proton exchange. Nat Biotechnol 25, 217–219 (2007). https://doi.org/10.1038/nbt1277
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DOI: https://doi.org/10.1038/nbt1277
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