Abstract
Erythroblast enucleation is thought to be largely dependent on signals mediated by other cells, such as macrophages. In an attempt to improve the in vitro production of red blood cells (RBCs) from immature hematopoietic progenitor cells, we have developed a method to produce enucleated RBCs efficiently in the absence of feeder cells. Our method may represent an efficient way to produce transfusable RBCs on a large scale from hematopoietic progenitors.
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Acknowledgements
We thank the Stem Cell Resource Network for providing cord blood.
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Contributions
K.M. designed and performed research and wrote the manuscript; T.H. and K.S. provided expertise; T.N. provided useful discussion; Y.N. coordinated research, analyzed data and wrote the manuscript.
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The authors declare no competing financial interests.
Supplementary information
Supplementary Fig. 1
Flow cytometry analysis (CD34+ cells). (PDF 60 kb)
Supplementary Fig. 2
Effect of vascular endothelial growth factor (VEGF) and insulin-like growth factor-II (IGF-II) on cell expansion in passage I. (PDF 50 kb)
Supplementary Fig. 3
Mean cell amplification. (PDF 46 kb)
Supplementary Fig. 4
Flow cytometry analysis (CD71 and Gly-A). (PDF 110 kb)
Supplementary Fig. 5
Morphology of the cells produced following passage III. (PDF 1499 kb)
Supplementary Fig. 6
Effect of MAP (mannitol, adenine, and phosphate) in passage IV (enucleation step). (PDF 4379 kb)
Supplementary Fig. 7
Flow cytometry analysis (Rh-D). (PDF 56 kb)
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Miharada, K., Hiroyama, T., Sudo, K. et al. Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells. Nat Biotechnol 24, 1255–1256 (2006). https://doi.org/10.1038/nbt1245
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