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Baculovirus solves a complex problem

An improved method for expressing multiple genes from a single baculovirus vector facilitates the production of protein complexes.

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Figure 1: Principle of rapid generation of multigene baculoviruses reported by Berger et al.

Bob Crimi


  1. 1

    Berger, I., Fitzgerald, D.J. & Richmond, T.J. Nat. Biotechnol. 22, 1583–1587 (2004).

    CAS  Article  Google Scholar 

  2. 2

    Smith, G.E., Summers, M.D. & Fraser, M.J. Mol. Cell Biol. 3, 2156–2165 (1983).

    CAS  Article  Google Scholar 

  3. 3

    Kitts, P.A., Ayres, M.D. & Possee, R.D. Nucleic Acids Res. 18, 5667–5672 (1990).

    CAS  Article  Google Scholar 

  4. 4

    Lalumiere, M. & Richardson, C.D. Methods Mol. Biol. 39, 161–177 (1995).

    CAS  PubMed  Google Scholar 

  5. 5

    Luckow, V.A., Lee, S.C., Barry, G.F. & Olins, P.O. J. Virol. 67, 4566–4579 (1993).

    CAS  Article  Google Scholar 

  6. 6

    Zhao, Y., Chapman, D.A. & Jones, I.M. Nucleic Acids Res. 31, E6–6 (2003).

    Article  Google Scholar 

  7. 7

    Emery, V.C. & Bishop, D.H. Protein Eng. 1, 359–366 (1987).

    CAS  Article  Google Scholar 

  8. 8

    Belyaev, A.S. & Roy, P. Nucleic Acids Res. 21, 1219–1223 (1993).

    CAS  Article  Google Scholar 

  9. 9

    Noad, R. & Roy P. Trends Microbiol. 11, 438–444 (2003).

    CAS  Article  Google Scholar 

  10. 10

    Ghosh, K. & Van Duyne, G.D. Cre-loxP biochemistry. Methods 28, 374–383 (2002).

    CAS  Article  Google Scholar 

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Roy, P. Baculovirus solves a complex problem. Nat Biotechnol 22, 1527–1528 (2004).

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