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A network of protein–protein interactions in yeast


A global analysis of 2,709 published interactions between proteins of the yeast Saccharomyces cerevisiae has been performed, enabling the establishment of a single large network of 2,358 interactions among 1,548 proteins. Proteins of known function and cellular location tend to cluster together, with 63% of the interactions occurring between proteins with a common functional assignment and 76% occurring between proteins found in the same subcellular compartment. Possible functions can be assigned to a protein based on the known functions of its interacting partners. This approach correctly predicts a functional category for 72% of the 1,393 characterized proteins with at least one partner of known function, and has been applied to predict functions for 364 previously uncharacterized proteins.

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Figure 1: (A) An interaction map of the yeast proteome assembled from published interactions.
Figure 2: Interactions between functional groups.
Figure 3: Interactions between proteins of different compartments.
Figure 4: Prediction of function by direct and indirect protein interactions.


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We thank Phil Green, Maynard Olson, Peer Bork, David Eisenberg, Trey Ideker, and members of the laboratory for helpful discussions and comments on the manuscript. This work has been supported by fellowships from the German Academic Exchange Service DAAD (to B.S. and P.U.) and grants from the National Institutes of Health (P41 RR11823) and The Institute for Systems Biology. S.F. is an investigator of the Howard Hughes Medical Institute.

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Correspondence to Benno Schwikowski, Peter Uetz or Stanley Fields.

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Schwikowski, B., Uetz, P. & Fields, S. A network of protein–protein interactions in yeast. Nat Biotechnol 18, 1257–1261 (2000).

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