Elastin-like polypeptides (ELPs) are pentapeptide oligomers that aggregate when heated above a critical temperature that is determined by the specific sequence of amino acids in the monomer. Meyer and Chilkoti have made use of this phenomenon to develop a strategy for the temperature-dependent purification of ELP-tagged recombinant proteins (see p. 1112). Their new system provides an inexpensive and simple alternative to chromatography-based purification schemes.
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Frederickson, R. Just heat and. . .purify. Nat Biotechnol 17, 1051 (1999). https://doi.org/10.1038/15019
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DOI: https://doi.org/10.1038/15019