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Recovery of homogeneous and functional β2-adrenergic receptors from extracellular baculovirus particles

Nature Biotechnology volume 15, pages 1300–1304 (1997)Cite this article

Abstract

Expression in haculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor purification steps. We show that vire particles released from Sf9 cells infected with a recombinant baculovirus coding for the human β2-adrenergic receptor (β2AR) cDNA contain glycosylated and biologically active β2AR. In addition, post-translational modifications known to modulate receptor activity were found to occur in these particles.

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Author information

Authors and Affiliations

  1. Département de Biochimie and Le Groupe de Recherche sur le Système Nerveux Autonome, Université de Montréal, PQ, H3C 3J7, Canada

    Thomas P. Loisel, Hervé Ansanay, Stéphane St-Onge & Michel Bouvier

  2. Laboratoire de Virologie et Pathogénesès Moléculaires (CNRS URA-1487), Faculté de Médecine, Institut de Biologie, 34060, Montpellier, France

    Bernard Gay & Pierre Boulanger

  3. Institut Cochin de Génétique Moléculaire, Laboratoire d'Immunopharmacologie Moléculaire, CNRS UPR 415 and University of Paris VII, 22, rueMéchain, 75014, Paris, France

    A. Donny Strosberg & Stefano Marullo

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  1. Thomas P. Loisel
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Loisel, T., Ansanay, H., St-Onge, S. et al. Recovery of homogeneous and functional β2-adrenergic receptors from extracellular baculovirus particles. Nat Biotechnol 15, 1300–1304 (1997). https://doi.org/10.1038/nbt1197-1300

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