Transgenic potato plants expressing mutant alleles of PLRV ORF4, the gene for the movement protein pr17 of this luteovirus, were generated for broad-range protection against virus infection. When tested for protection against infection by PLRV, all transgenic lines showed a significant reduction of virus antigen. Potato lines accumulating N- or C-terminally extended PLRV pr17 mutant proteins were resistant to infection by the unrelated potato viruses PVY and PVX. Transgenic lines that did not express protein despite high transcript levels failed to exhibit virus resistance.
Subscribe to Journal
Get full journal access for 1 year
only $20.83 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Ross, H. 1986. Potato Breeding—Problems and Perspectives. Veriag Paul Parey, Berlin and Hamburg.
Lomonossof, G.P. 1995. Pathogen-derived resistance to plant viruses. Annu. Rev. Phytopath. 33: 323–343.
Cooper, B., Lapidot, M., Heick, J.A., Dodds, J.A., and Beachy, R.N. 1995. A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analogue increases susceptibility. Virology 206: 307–313.
Tacke, E., Prüfer, D., Schmitz, J., and Rohde, W. 1991. The potato leafroll luteovirus 17K protein is a single-stranded nucleic acid-binding protein. J. Gen. Virol. 72: 2035–2038.
Tacke, E., Schmitz, J., Prüfer, D., and Rohde, W. 1993. Mutational analysis of the nucleic acid-binding 17kDa phosphoprotein of potato leafroll luteovirus identifies an amphipathic α-helix as the domain for protein/protein interactions. Virology 197: 274–282.
Schmitz, J. 1995. Molekularbiologische Studien am vermuteten Transportprotein des potato teafroll virus (PLRV). Untersuchungen zur Lokalisation und alternativen Expression von pr17. Ph.D. diss., University of Cologne, Germany.
Chay, C.A., Gunasinge, U.B., Dinesh-Kumar, S.R., Miller, W.A., and Gray, S. 1996. Aphid transmission and systemic plant infection determinants of barley yellow dwarf luteovirus-PAV are contained in the coat protein readthrough domain and 17-kDa, respectively. Virology 219: 57–65.
Miller, W.A., Dinesh-Kumar, S.P., and Paul, C.P. 1995. Luteovirus gene expression. Crit. Rev. Plant Sci. 14: 179–211.
Lütcke, H.A., Chow, K.C., Mickel, F.S., Moss, K.A., Kern, H.F., and Scheele, G.A. 1987. Selection of AUG initiation codons differs in plants and animals. EMBO J. 6: 43–48.
Bol, J.F., Linthorst, H.J.M., and Cornelissen, B.J.C. 1990. Plant pathogenesis-related proteins induced by virus infection. Annu. Rev. Phytopathol. 28: 113–138.
Tacke, E., Salamini, F., and Rohde, W. 1996. Genetic engineering of potato for resistance to potato leafroll virus (PLRV) infection. Proceedings of the Ninth EAPR Virology Section Meet., pp. 15–22.
Lapidot, M., Gafny, R., Ding, B., Wolf, S., Lucas, W.J., and Beachy, R.N. 1993. A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plants. Plant J. 4: 959–970.
Malyshenko, S.I., Kondokova, S.I., Nazarova, Ju.V., Kaplan, I.B., Taliansky, M.E., and Atabekov, J.G. 1993. Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport protein. J. Gen. Virol. 74: 1149–1156.
van der Wilk, F., Willink, D.R-L., Huisman, M.J., Huttinga, H., and Goldbach, R. 1991. Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Mol. Biol. 17: 431–439.
Barker, H., Reavy, B., Webster, K.D., Jolly, C.A., Kumar, A., and Mayo, M.A. 1993. Relationship between transcript production and virus resistance in transgenic tobacco expressing the potato leafroll virus coat protein gene. Plant Cell Rep. 13: 54–58.
Kawchuk, L.M., Martin, R.R., and McPherson, J. 1991. Sense and antisense RNA-mediated resistance to potato leafroll virus in Russet Burbank potato plants. Mol. Plant-Microbe Interact. 4: 247–253.
Schmitz, J., Prüfer, D., Rohde, W., and Tacke, E. 1996. Non-canonical translation mechanisms in plants: efficient in vitro and in planta initiation at AUU codons of the tobacco mosaic virus enhancer sequence. Nucleic Acids Res. 24: 257–263.
Tacke, E., Kull, B., Prüfer, D., Reinold, S., Schmitz, J., Salamini, F., et al. 1995. PLRV gene expression in potato, pp. 353–367 in Biotechnology and Plant Protection. Bills, D.D. and Kung., S.-D. (eds.). World Scientific Publishing, Singapore.
Töpfer, R., Matzeit, V., Gronenbom, B., Schell, J., and Steinbiss, H.H. 1987. A set of plant expression vectors fortranscriptkmal and translational fusions. Nucleic Acids Res. 15: 5890.
Bevan, M. 1984. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12: 8711–8721.
Tacke, E., Korfhage, C., Michel, D., Maddaloni, M., Motto, M., et al. 1995. Transposon tagging of the maize Glossy2 locus with the transposable element En/Spm . Plant J. 8: 907–917.
Tacke, E., Prüfer, D., Salamini, F., and Rohde, W. 1990. Characterization of a potato leafroll luteovirus subgenomic RNA: differential expression by internal translation initiation and UAG suppression. J. Gen Virol. 71: 2265–2272.
Strittmatter, G., Gheysen, G., Gianinazzi-Pearson, V., Hahn, K., Niebel, A., Rohde, W., and Tacke, E. 1996. Infections with various types of organisms stimulate transcription from a short promoter fragment of the potato gst1 gene. Mol. Plant-Microbe Interact. 9: 68–73.
Qerci, M., Baulcombe, D.C., Goldbach, R.W., and Salazar, L.F. 1995. Analysis of resistance-breaking determinants of potato virus X (PVX) HB on different potato genotypes expressing extreme resistance to PVX. Phytopathology 85: 1003–1010.
About this article
Cite this article
Tacke, E., Salamini, F. & Rohde, W. Genetic engineering of potato for broad-spectrum protection against virus infection. Nat Biotechnol 14, 1597–1601 (1996). https://doi.org/10.1038/nbt1196-1597
Biotechnological advancement in genetic improvement of broccoli (Brassica oleracea L. var. italica), an important vegetable crop
Biotechnology Letters (2016)
Biotechnological applications in in vitro plant regeneration studies of broccoli (Brassica oleracea L. var. italica), an important vegetable crop
Biotechnology Letters (2016)
Computational sequence analysis of predicted long dsRNA transcriptomes of major crops reveals sequence complementarity with human genes
GM Crops & Food (2013)
Evaluation of a wide range of pepper genotypes for regeneration and transformation with an Agrobacterium tumefaciens shooter strain
South African Journal of Botany (2008)