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Transposition Mediated Re–positioning and Subsequent Elimination of Marker Genes from Transgenic Tomato

Nature Biotechnology volume 11, pages 12861292 (1993) | Download Citation

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Abstract

We describe a new plant transformation vector system which utilizes the transposition functions of the maize Ac/Ds transposable element family to re-position transgenes in transgenic crop plants. The practical applications of the system are two-fold. It allows the production of plants which exhibit a range of different stabilizable transgene expression levels following a single primary transformation event, and it allows for the elimination of specific transgene sequences—such as a selectable marker gene—subsequent to the transformation event. We have demonstrated the system using the NptII selectable marker gene and a Ds element containing the GUS reporter gene. Progeny plants were recovered from primary transformants from which either the NptII gene or the Ds/GUS element have been eliminated. We also show that the expression level of the GUS gene within both individual and amplified Ds elements can vary as a function of their position in the genome following transposition.

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Author information

Author notes

    • John I. Yoder

    Corresponding author.

Affiliations

  1. Department of Vegetable Crops, University of California, Davis, Davis, CA 95616.

    • Cleofe N. Lastrella
    •  & John I. Yoder
  2. PBI Cambridge, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK.

    • Andrew P. Goldsbrough

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DOI

https://doi.org/10.1038/nbt1193-1286

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