Research Paper | Published:

Trypanosoma Cruzi Flagellar Repetitive Antigen Expression by Recombinant Baculovirus: Towards an Improved Diagnostics Reagent for Chagas' Disease

Bio/Technologyvolume 10pages14741477 (1992) | Download Citation

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Abstract

We constructed a recombinant baculovirus that expressed part of a Trypanosorna cruzi flagellar repetitive antigen (FRA). Both cell- associated and secreted forms of recombinant FRA were detected in cultures of virus-infected Spodoptera frugiperda (Sf9) cells. These forms show a complex pattern after polyacrylamide gel electrophoresis and Western blot analysis using either an anti-FRA rabbit serum or human Chagasic sera. Competitive Western-blot experiments revealed that all bands react with the same antibodies as a bacterially-derived FRA. Polymerase chain reaction and Southern blots of the recombinant viral DNA also showed a complex pattern, suggesting the presence of more than one repeat unit in the viral genome. When tested against a panel of human sera from an endemic area for Chagas' disease, FRA recombinant-Sf9 culture supernatant showed the same reactivity as purified FRA produced in bacteria.

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Author notes

  1. Claudia N. Duarte dos Santos: Corresponding author.

Affiliations

  1. Department of Biochemistry and Molecular Biology, Inst. Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, Brazil, 21045

    • Claudia N. Duarte dos Santos
    • , Marco A. Krieger
    • , Elza Almeida
    • , Juan J. Lafaille
    • , Samuel Goldenberg
    •  & Ricardo Galler

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https://doi.org/10.1038/nbt1192-1474

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