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“Primatization” of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4

Bio/Technology volume 10, pages 1455–1460 (1992)Cite this article

Abstract

Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%–98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys immunized with human CD4 antigen and a human/monkey chimeric anti-CD4 antibody with 91–92% homology to human immunoglobulin framework regions was cloned and expressed. The antibody has an apparent affinity of 3.2 × 10−11 M and exhibits potent immunosuppressive properties in vitro.

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Author notes

  1. Roland Newman: Corresponding author.

Authors and Affiliations

  1. IDEC Pharmaceuticals Corporation, 11099 North Torrey Pines Rd., La Jolla, CA, 92037

    Roland Newman, James Alberts, Darrell Anderson, Kristin Carner, Cheryl Heard, Frank Norton, Ron Raab, Mitchell Reff, Steve Shuey & Nabil Hanna

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  3. Darrell Anderson
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Newman, R., Alberts, J., Anderson, D. et al. “Primatization” of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4. Nat Biotechnol 10, 1455–1460 (1992). https://doi.org/10.1038/nbt1192-1455

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