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Rescuing Transgene Expression by Co-Integration

Abstract

To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep β-lactoglobulin gene with two poorly expressed β-lactoglobulin-derived genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. “Rescuing” transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.

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Clark, A., Cowper, A., Wallace, R. et al. Rescuing Transgene Expression by Co-Integration. Nat Biotechnol 10, 1450–1454 (1992). https://doi.org/10.1038/nbt1192-1450

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