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Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase

Bio/Technology volume 9, pages 1096–1099 (1991)Cite this article

Abstract

We have shown that the isolated cellulose binding domain of endoglucanase A (GenA) from the bacterium Cellulomonas fimi disrupts the structure of cellulose fibres and releases small particles but has no detectable hydrolytic activity. In contrast, the isolated catalytic domain of this enzyme does not disrupt the fibril structure but polishes the surface of the fibre concomitant with the release of reducing sugars.

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Authors and Affiliations

  1. Department of Microbiology, University of British Columbia, 300-6174 University Boulevard, Vancouver, B.C., V6T 1Z3, Canada

    Neena Din, Neil R. Gilkes, Bahar Tekant, Robert C. Miller Jr., R. Anthony J. Warren & Douglas G. Kilburn

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  1. Neena Din
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  2. Neil R. Gilkes
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  3. Bahar Tekant
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  4. Robert C. Miller Jr.
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  5. R. Anthony J. Warren
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  6. Douglas G. Kilburn
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Din, N., Gilkes, N., Tekant, B. et al. Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase. Nat Biotechnol 9, 1096–1099 (1991). https://doi.org/10.1038/nbt1191-1096

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