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Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.

Bio/Technology volume 8, pages 1041–1045 (1990)Cite this article

Abstract

Grapes, the world's most widely-grown fruit crop, are difficult to breed by conventional methods. Here we report a first step in the application of biotechnology to the genetic improvement of this crop, the development of methods for genetic transformation. Co-cultivation of hypocotyl ex-plants from somatic embryos of the root-stock, Rupestris St. George (Vitis rupestris), with a disarmed Agrobacterium tumefaciens strain encoding β-glucuronidase (GUS) and kanamycin resistance, followed by culture on a regeneration medium containing kanamycin, led to formation of transgenic buds and transgenic plants. Petiole explants of the Vitis vinifera cultivars, Cabernet Sauvignon and Ghardonnay, treated similarly gave only transgenic buds.

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Authors and Affiliations

  1. Department of Viticulture and Enology, University of California, Davis, CA, 95616

    Michael G. Mullins & F. C. Archie Tang

  2. Calgene, 1920 Fifth Street, Davis, CA, 95616

    Daniel Facciotti

Authors
  1. Michael G. Mullins
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  2. F. C. Archie Tang
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  3. Daniel Facciotti
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Mullins, M., Tang, F. & Facciotti, D. Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.. Nat Biotechnol 8, 1041–1045 (1990). https://doi.org/10.1038/nbt1190-1041

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