Eukaryotic host cells co-transfected with a human growth hormone gene (hGH) under the control of the promoter of the human 70,000 Mr heat shock protein (hsp70) gene, and with the human harvey ras oncogen, were expanded as tumours after inoculation into selected warmblooded animals. Upon stressing the animals, employing controlled whole body heat, gene expression occurs, and hGH protein can be detected in the blood stream of the animals. In addition, in response to the secretion of the newly synthesized protein after induction, non-genetically immunodeficient animals produce antibodies over a period of four weeks, able to compete monoclonal antibodies, or to immunoprecititate the hGH produced by the parental cells. This approach represents a simple method for the direct production in vivo of a given gene product, as well as polyclonal antibodies directed against it. Moreover, it offers a general method of primary immunization with an engineered gene product.
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Dreano, M., Marq, J. & Bromley, P. Antibody Formation Against Heat-Induced Gene Products Expressed in Animals. Nat Biotechnol 6, 1340–1343 (1988). https://doi.org/10.1038/nbt1188-1340
Journal of Biotechnology (1992)