Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens

Abstract

Here we describe a strategy for engineering transgenic plants with broad-spectrum resistance to bacterial and fungal phytopathogens. We expressed a synthetic gene encoding a N terminus-modified, cecropin–melittin cationic peptide chimera (MsrA1), with broad-spectrum antimicrobial activity. The synthetic gene was introduced into two potato (Solanum tuberosum L.) cultivars, Desiree and Russet Burbank, stable incorporation was confirmed by PCR and DNA sequencing, and expression confirmed by reverse transcription (RT)-PCR and recovery of the biologically active peptide. The morphology and yield of transgenic Desiree plants and tubers was unaffected. Highly stringent challenges with bacterial or fungal phytopathogens demonstrated powerful resistance. Tubers retained their resistance to infectious challenge for more than a year, and did not appear to be harmful when fed to mice. Expression of msrA1 in the cultivar Russet Burbank caused a striking lesion-mimic phenotype during leaf and tuber development, indicating its utility may be cultivar specific. Given the ubiquity of antimicrobial cationic peptides as well as their inherent capacity for recombinant and combinatorial variants, this approach may potentially be used to engineer a range of disease-resistant plants.

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Figure 1: The structure and expression constructs for MsrA1.
Figure 2: msrA1 gene integration and mRNA expression.
Figure 3: Morphological characteristics of transgenic potato plants and tubers.
Figure 4: Transgenic potato challenged with the fungal pathogen Phytophthora cactorum.
Figure 5: Transgenic potato challenged with the fungal pathogen Fusarium solani.
Figure 6: Transgenic potatoes resistant to Erwinia carotovora.

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Acknowledgements

This work was funded by the Natural Sciences and Engineering Research Council (NSERC) Strategic grant to S.M. and W.W.K. We thank N. Vettakorrumkankav, L. Sun, X. Yu, and T. Stevenson for expert technical assistance, and Dr. Zamir Punja (Simon Fraser University, Burnaby, BC, Canada) for providing fungal phytopathogens.

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Correspondence to William W. Kay or Santosh Misra.

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Osusky, M., Zhou, G., Osuska, L. et al. Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat Biotechnol 18, 1162–1166 (2000). https://doi.org/10.1038/81145

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