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Engineering antibody Fv fragments for cancer detection and therapy: Bisulfide-stabilized Fv fragments

Nature Biotechnologyvolume 14pages12391245 (1996) | Download Citation

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Abstract

Disulfide-stabilized Fv fragments of antibodies (dsFv) are molecules in which the VH-VL heterodimer is stabilized by an interchain disulfide bond engineered between structurally conserved framework positions distant from complementarity-determining regions (CDRs). This method of stabilization is applicable for the stabilization of many antibody Fvs and has also been applied to a T-cell receptor Fv. A summary of the design strategy, and the construction and production of various dsFvs and dsFv-fusion proteins is presented. Included in the discussion are the biochemical features of dsFvs in comparison with scFvs, the effect of disulfide stabilization on Fv binding and activity, and various applications of dsFvs and dsFv-immunotoxins for tumor imaging and the treatment of solid tumors in animal models.

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  1. Ira Pastan: Corresponding author.

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  1. Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 37 Convent Drive MSC 4255, Bethesda, MD, 20892-4255

    • Yoram Reiter
    • , Ulrich Brinkmann
    • , Byungkook Lee
    •  & Ira Pastan

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https://doi.org/10.1038/nbt1096-1239

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