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Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library

Bio/Technology volume 11, pages 1145–1149 (1993)Cite this article

Abstract

We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective hi assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.

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Author information

Author notes

  1. James D. Marks

    Present address: Department of Anesthesia, University of California, San Francisco General Hospital, San Francisco, California, 94110

  2. Willem H. Ouwehand: Corresponding author.

Authors and Affiliations

  1. MRC Centre for Protein Engineering, Hills Road, Cambridge, CB2 2QH, UK

    James D. Marks & Greg Winter

  2. Division of Transfusion Medicine, East Anglian Blood Transfusion Centre, University of Cambridge, Long Road, Cambridge, CB2 2PT, UK

    Willem H. Ouwehand, Ricarda Finnern & Douglas Voak

  3. Division of Haematology, National Institute for Biological Standards and Control, Blanche Lane, Potters Bar, EN6 3QG, UK

    Willem H. Ouwehand & Susan Thorpe

  4. MRC Immunopathology Unit, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK

    Jacqueline M. Bye, Barbara D. Gorick & Nevin C. Hughes-Jones

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Marks, J., Ouwehand, W., Bye, J. et al. Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library. Nat Biotechnol 11, 1145–1149 (1993). https://doi.org/10.1038/nbt1093-1145

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