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Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library

Bio/Technologyvolume 11pages11451149 (1993) | Download Citation



We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective hi assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.

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Author notes

    • James D. Marks

    Present address: Department of Anesthesia, University of California, San Francisco General Hospital, San Francisco, California, 94110

  1. Willem H. Ouwehand: Corresponding author.


  1. MRC Centre for Protein Engineering, Hills Road, Cambridge, CB2 2QH, UK

    • James D. Marks
    •  & Greg Winter
  2. Division of Transfusion Medicine, East Anglian Blood Transfusion Centre, University of Cambridge, Long Road, Cambridge, CB2 2PT, UK

    • Willem H. Ouwehand
    • , Ricarda Finnern
    •  & Douglas Voak
  3. Division of Haematology, National Institute for Biological Standards and Control, Blanche Lane, Potters Bar, EN6 3QG, UK

    • Willem H. Ouwehand
    •  & Susan Thorpe
  4. MRC Immunopathology Unit, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK

    • Jacqueline M. Bye
    • , Barbara D. Gorick
    •  & Nevin C. Hughes-Jones


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