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Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library


We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective hi assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.

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Marks, J., Ouwehand, W., Bye, J. et al. Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library. Nat Biotechnol 11, 1145–1149 (1993).

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