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Characterization of RNA–Mediated Resistance to Tomato Spotted Wilt Virus in Transgenic Tobacco Plants

Bio/Technology volume 10, pages 1133–1137 (1992)Cite this article

Abstract

Recently high levels of protection against tomato spotted wilt virus (TSWV), a negative–strand RNA virus infecting plants, have been obtained by transforming tobacco with viral nucleoprotein (N) gene sequences. Here we demonstrate that this protection is primarily due to the presence of N gene transcripts in the cells of transgenic plants, and hence appears to be RNA–mediated. Further, transgenic tobacco plants are only protected to isolates and strains of TSWV and not to other tospoviruses that share considerable nucleotide sequence homology in their N genes to TSWV. In addition to being protected after mechanical inoculation, the transgenic tobacco plants are also resistant to inoculation using viruliferous thrips, i.e. Frankliniella occidentalis (Perg.), one of the most important natural vector species.

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  1. Peter de Haan: Corresponding author.

Authors and Affiliations

  1. Department of Virology, Agricultural University Wageningen, Binnenhaven 11, P.O. Box 8045, 6700 EM, Wageningen, The Netherlands

    Marcel Prins, Ineke G. Wijkamp, Dick Peters & Rob Goldbach

  2. Zaadunie B.V., Department of Biotechnology, Westeinde 62, P.O. Box 26, 1600 AA, Enkhuizen, The Netherlands

    Peter de Haan, Jan J. L. Gielen, Alie van Schepen & Mart Q. J. M. van Grinsven

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  1. Peter de Haan
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Haan, P., Gielen, J., Prins, M. et al. Characterization of RNA–Mediated Resistance to Tomato Spotted Wilt Virus in Transgenic Tobacco Plants. Nat Biotechnol 10, 1133–1137 (1992). https://doi.org/10.1038/nbt1092-1133

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