Research Note | Published:

Efficient Transformation of Tobacco by Ultrasonication

Bio/Technology volume 9, pages 996997 (1991) | Download Citation

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Abstract

We report a simple, direct gene transfer technique based on sonication. Transient expression of a β–glucoronidase (GUS) gene was detected in 86 percent of treated leaf segments of Nicotiana tabacum, and the area of transformed tissue on these segments reached 60–70 percent. GUS activity in the leaves of regenerated, kanamycin resistant plantlets was found at a frequency of 22 percent, and analysis of R1 regenerated seedlings showed that both kanamycin resistance and GUS behaved as typical linked dominant genes.

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Author information

Author notes

    • Shi-Rong Jia

    Corresponding author.

Affiliations

  1. Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

    • Li-Jian Zhang
    • , Le-Mei Cheng
    • , Chang-Gong Li
    • , Jing Yuan
    •  & Shi-Rong Jia
  2. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.

    • Ning Xu
    •  & Nan-Ming Zhao

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DOI

https://doi.org/10.1038/nbt1091-996

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