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Abstract

We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.

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Author notes

    • Nigel S. Dunn-Coleman

    Corresponding author

Affiliations

  1. Genencor International Inc., 180 Kimball Way, South San Francisco, CA 94080.

    • Nigel S. Dunn-Coleman
    • , Peggy Bloebaum
    • , Randy M. Berka
    • , Elizabeth Bodie
    • , Nancy Robinson
    • , Gale Armstrong
    • , Michael Ward
    • , Melinda Przetak
    • , Gina L. Carter
    • , Regina LaCost
    • , Lori J. Wilson
    • , Katherine H. Kodama
    • , Enrique F. Baliu
    • , Ben Bower
    • , Michael Lamsa
    •  & Henry Heinsohn

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DOI

https://doi.org/10.1038/nbt1091-976