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Commercial Levels of Chymosin Production by Aspergillus

Bio/Technology volume 9, pages 976–981 (1991)Cite this article

Abstract

We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.

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Author notes

  1. Nigel S. Dunn-Coleman: Corresponding author

Authors and Affiliations

  1. Genencor International Inc., 180 Kimball Way, South San Francisco, CA, 94080

    Nigel S. Dunn-Coleman, Peggy Bloebaum, Randy M. Berka, Elizabeth Bodie, Nancy Robinson, Gale Armstrong, Michael Ward, Melinda Przetak, Gina L. Carter, Regina LaCost, Lori J. Wilson, Katherine H. Kodama, Enrique F. Baliu, Ben Bower, Michael Lamsa & Henry Heinsohn

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  1. Nigel S. Dunn-Coleman
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  2. Peggy Bloebaum
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  3. Randy M. Berka
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  4. Elizabeth Bodie
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  5. Nancy Robinson
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  6. Gale Armstrong
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  7. Michael Ward
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  8. Melinda Przetak
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  9. Gina L. Carter
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  10. Regina LaCost
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  11. Lori J. Wilson
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  12. Katherine H. Kodama
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  13. Enrique F. Baliu
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  14. Ben Bower
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  15. Michael Lamsa
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  16. Henry Heinsohn
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Dunn-Coleman, N., Bloebaum, P., Berka, R. et al. Commercial Levels of Chymosin Production by Aspergillus. Nat Biotechnol 9, 976–981 (1991). https://doi.org/10.1038/nbt1091-976

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