Research Paper | Published:

Cloning and Expression of a DNA Sequence Conferring Cephamycin C Production

Bio/Technologyvolume 6pages12221224 (1988) | Download Citation

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Abstract

Cephamycin C, a member of the 7-methoxycephalosporin family of β-lactam antibiotics, is widely produced among actinomycetes. A 29.3 kb DNA segment from Streptomyces cattleya NRRL8057 was cloned that directed cephamycin C production in a heterologous host, Streptomyces lividans 1326. Introduction of the gene cluster into another cephamycin C-producing Streptomyces increased the production of cephamycin C.

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Author notes

    • Carton W. Chen

    Present address: Institute of Microbiology and Immunology,

    • Jane F. -Y. Tsai

    Present address: The Rockefeller University, 1260 York Ave., New York City, NY, 10021, USA

  1. Carton W. Chen: Corresponding author.

Affiliations

  1. Panlabs Fermtech Division, Panlabs Taiwan, Ltd., P.O. Box 26-127, Taipei, Taiwan, ROC

    • Hsiu-Fen Lin
    • , Cynthia L. Kuo
    •  & Hsiu-Lan Tsai
  2. Institute of Genetics, National Yang-Ming Medical College, Taipei, Taiwan, ROC

    • Carton W. Chen

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DOI

https://doi.org/10.1038/nbt1088-1222

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