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Cloning and Expression of a DNA Sequence Conferring Cephamycin C Production

Bio/Technology volume 6, pages 1222–1224 (1988)Cite this article

Abstract

Cephamycin C, a member of the 7-methoxycephalosporin family of β-lactam antibiotics, is widely produced among actinomycetes. A 29.3 kb DNA segment from Streptomyces cattleya NRRL8057 was cloned that directed cephamycin C production in a heterologous host, Streptomyces lividans 1326. Introduction of the gene cluster into another cephamycin C-producing Streptomyces increased the production of cephamycin C.

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Author notes

  1. Carton W. Chen

    Present address: Institute of Microbiology and Immunology,

  2. Jane F. -Y. Tsai

    Present address: The Rockefeller University, 1260 York Ave., New York City, NY, 10021, USA

  3. Carton W. Chen: Corresponding author.

Authors and Affiliations

  1. Panlabs Fermtech Division, Panlabs Taiwan, Ltd., P.O. Box 26-127, Taipei, Taiwan, ROC

    Hsiu-Fen Lin, Cynthia L. Kuo & Hsiu-Lan Tsai

  2. Institute of Genetics, National Yang-Ming Medical College, Taipei, Taiwan, ROC

    Carton W. Chen

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  4. Hsiu-Lan Tsai
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  5. Jane F. -Y. Tsai
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Chen, C., Lin, HF., Kuo, C. et al. Cloning and Expression of a DNA Sequence Conferring Cephamycin C Production. Nat Biotechnol 6, 1222–1224 (1988). https://doi.org/10.1038/nbt1088-1222

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  • DOI: https://doi.org/10.1038/nbt1088-1222

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