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Rapid Production of Transgenic Flowering Shoots and F1 Progeny from Nicotiana plumbaginifolia Epidermal Peels

Bio/Technology volume 5, pages 1081–1084 (1987)Cite this article

An Erratum to this article was published on 01 December 1987

Abstract

Epidermal segments derived from flowering branches of Nicotiana plumbaginifolia were co-cultivated with a disarmed strain of Agrobacterium tumefaciens containing the co-integrate pTiBGSSSE∷pMONC9. Inoculated segments were subsequently grown on a medium for flower induction, and transgenic flowering shoots were recovered at a frequency of 40 percent. The transgenic plants obtained in vitro expressed the rbcS-8B-CAT gene in the appropriate organs. The flowers were fertile and set seeds which germinated with close to 100 percent frequency to give transgenic F1 progeny. Analysis of the kanamycin-resistant marker and CAT activity demonstrated that the transgenes were inherited as dominant Mendelian traits. With this technique it is possible to obtain transgenic plants with flowers and seeds six and eight weeks, respectively, after inoculation with Agrobacterium.

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Author notes

  1. Seth Mante

    Present address: Appalachian Fruit Research Station, Route 2, Box 45, Kearnysville, West Virginia, 25430

Authors and Affiliations

  1. Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York, 10021–6399

    Toan Hanh Trinh & Nam-Hai Chua

  2. Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Republic of Singapore, 0511

    Eng-Chong Pua

Authors
  1. Toan Hanh Trinh
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  2. Seth Mante
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  3. Eng-Chong Pua
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  4. Nam-Hai Chua
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Hanh Trinh, T., Mante, S., Pua, EC. et al. Rapid Production of Transgenic Flowering Shoots and F1 Progeny from Nicotiana plumbaginifolia Epidermal Peels. Nat Biotechnol 5, 1081–1084 (1987). https://doi.org/10.1038/nbt1087-1081

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  • DOI: https://doi.org/10.1038/nbt1087-1081

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