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In vivo detection of amyloid-β deposits by near-infrared imaging using an oxazine-derivative probe

Nature Biotechnologyvolume 23pages577583 (2005) | Download Citation



As Alzheimer's disease pathogenesis is associated with the formation of insoluble aggregates of amyloid β-peptide, approaches allowing the direct, noninvasive visualization of plaque growth in vivo would be beneficial for biomedical research. Here we describe the synthesis and characterization of the near-infrared fluorescence oxazine dye AOI987, which readily penetrates the intact blood-brain barrier and binds to amyloid plaques. Using near-infrared fluorescence imaging, we demonstrated specific interaction of AOI987 with amyloid plaques in APP23 transgenic mice in vivo, as confirmed by postmortem analysis of brain slices. Quantitative analysis revealed increasing fluorescence signal intensity with increasing plaque load of the animals, and significant binding of AOI987 was observed for APP23 transgenic mice aged 9 months and older. Thus, AOI987 is an attractive probe to noninvasively monitor disease progression in animal models of Alzheimer disease and to evaluate effects of potential Alzheimer disease drugs on the plaque load.

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The authors would like to thank Alexandra Suter for her excellent technical assistance.

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  1. Discovery Technologies, Novartis Institutes for Biomedical Research, Basel, CH-4002, Switzerland

    • Martin Hintersteiner
    • , Willy Kinzy
    • , Rainer Kneuer
    • , Markus Rudin
    • , Markus Stoeckli
    •  & Hans-Ulrich Gremlich
  2. Nervous System Department, Novartis Institutes for Biomedical Research, Basel, CH-4002, Switzerland

    • Albert Enz
    • , Peter Frey
    • , Anne-Lise Jaton
    • , Ulf Neumann
    • , Matthias Staufenbiel
    •  & Karl-Heinz Wiederhold


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The authors declare no competing financial interests.

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Correspondence to Hans-Ulrich Gremlich.

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