Genetic instability is common in commercial strains of Streptomyces. Streptomyces cattleya segregates Arg− mutants at high frequency. The argG gene from wild type S. cattleya was cloned by complementation of argG in E. coli. DNA containing argG was used as a probe in Southern blot analysis of S. cattleya strains. Arg− mutants of S. cattleya have deleted the region of DNA encoding the argG gene.
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Redshaw, P.A., McCann, P.A., Sankaran, L. and Pogell, B.M. 1976. Control of differentiation in Streptomycetes: involvement of extrachromosomal deoxyribonucleic acid and glucose repression in aerial mycelia development. J. Bacteriol. 125: 698–705.
Pogell, B. 1979. Regulation of aerial mycelium formation in Streptomyces, p. 218–224. In: Genetics of Industrial Microorganisms. Sebek, O. and Laskin, A. (eds.), A.S.M., Washington, D.C.
Altenbuchner, J. and Cullum, J. 1984. DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Mol. Gen. Genet. 195: 134–138.
Kahan, J.S., Kahan, F.M., Goegelman, R., Currie, S.A., Jackson, M., Stapley, E.O., Miller, T.W., Miller, A.K., Hendlin, D., Mochales, S., Hernandez, S., Woodruff, H.D. and Birnbaum, J. 1979. Thienamycin, a new beta-lactam antibiotic I. discovery, taxonomy, isolation and physical properties. The Journal of Antibiotics, 32: 1–12.
Bolivar, F., Rodriguez, R., Greene, P., Betlach, M., Heyneker, H. and Boyer, H. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95–113.
Bochner, B., Huang, H.C., Shieven, G. and Ames, B.N. 1980. Positive selections for loss of tetracycline resistance. J. Bacteriol. 143: 926–933.
Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Cohen, S.N., Chang, A.C.H. and Hsu, L. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R-factor DNA. Proc. Natl. Acad. Sci. USA 69: 2110–2114.
Clewell, D.B. and Helinski, D.R. 1979. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry 9: 4440–4482.
Chater, K.F., Hopwood, D.A., Kieser, T. and Thompson, C.J. 1982. Gene cloning in Streptomyces. Curr. Top. Microbiol. Immunol. 96: 69–95.
Hohn, B. and Collins, J. 1980. A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291–298.
Schrempf, H. 1983. Deletion and amplification of DNA sequences in melanin-negative variants of Streptomyces reticuli. Mol. Gen. Genet. 183: 501–505.
Fishman, S. and Hershberger, C. 1983. Amplified DNA in Streptomyces fradiae. J Bacteriol. 155: 459–66.
Schrempf, H. 1982. Plasmid loss and changes within the chromosomal DNA of Streptomyces reticuli. J. Bacteriol. 151: 701–707.
Maniatis, T., Fritsch, E. and Sambrook, J. 1982. Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Southern, E. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.
Thomas, P. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77: 5201–5205.
Rigby, P.W.J., Dieckman, M., Rhodes, C. and Berg, P. 1977. Labeling deoxyribonucleic acids to high specific activity in vitro by nick translation with DNA polymerase. J. Mol. Biol. 113: 237–251.
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Meade, H. Cloning of argG from Streptomyces: Loss of Gene in Arg− Mutants of S. cattleya. Nat Biotechnol 3, 917–918 (1985) doi:10.1038/nbt1085-917
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