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Cloning of argG from Streptomyces: Loss of Gene in Arg− Mutants of S. cattleya

Bio/Technology volume 3, pages 917–918 (1985)Cite this article

Abstract

Genetic instability is common in commercial strains of Streptomyces. Streptomyces cattleya segregates Arg− mutants at high frequency. The argG gene from wild type S. cattleya was cloned by complementation of argG in E. coli. DNA containing argG was used as a probe in Southern blot analysis of S. cattleya strains. Arg− mutants of S. cattleya have deleted the region of DNA encoding the argG gene.

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Authors and Affiliations

  1. Fermentation Microbiology Dept., Merck & Co., Inc., Rahway, NJ, 07065

    Harry Meade

  2. Biogen Research Corp., 14 Cambridge Center, Cambridge, MA, 02142

    Harry Meade

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  1. Harry Meade
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Meade, H. Cloning of argG from Streptomyces: Loss of Gene in Arg− Mutants of S. cattleya. Nat Biotechnol 3, 917–918 (1985). https://doi.org/10.1038/nbt1085-917

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  • DOI: https://doi.org/10.1038/nbt1085-917

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