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Automation of DNA Sequencing: A System to Perform the Sanger Dideoxysequencing Reactions

Bio/Technology volume 3, pages 911–915 (1985)Cite this article

Abstract

The determination of the complete nucleotide sequence of large DNA molecules is a tedious and time-consuming process. The repetitive nature of the task makes it an ideal subject for automation. We report here a system designed and constructed to perform the chain termination reactions of the Sanger sequencing procedure automatically. It is capable of carrying out these reactions for from five to twelve DNA templates at one time. The system takes 12 minutes to dispense 12 templates, add the reagents and mix them; the complete cycle time, including incubations, is 45 minutes.

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Author notes

  1. R. Wayne Davies

    Present address: Allelix Inc., 6850 Goreway Drive, Mississauga, Ontario, L4V 1P1, Canada

  2. William J. Martin, Bohdan R. Galinski, Michael Gallagher and Maurice S. Beck: Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD, U.K.

  3. John R. Warmington, R. Wayne Davies and Stephen G. Oliver: Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD, U.K.

  4. William J. Martin: Author to whom correspondence should be sent.

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    1. William J. Martin
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    2. John R. Warmington
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    3. Bohdan R. Galinski
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    4. Michael Gallagher
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    5. R. Wayne Davies
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    6. Maurice S. Beck
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    7. Stephen G. Oliver
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    Martin, W., Warmington, J., Galinski, B. et al. Automation of DNA Sequencing: A System to Perform the Sanger Dideoxysequencing Reactions. Nat Biotechnol 3, 911–915 (1985). https://doi.org/10.1038/nbt1085-911

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