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Microfiltration Culture Process for Enhanced Production of rDNA Receptor Cells of Escherichia Coli

Bio/Technology volume 2, pages 891–896 (1984)Cite this article

Abstract

A novel microfiltration batch culture process, suitable for scale-up, has been designed, instrumented, and studied with a representative recombinant-DNA receptor strain of Escherichia coli. In this process nutrient medium is fed continuously into an aerated and stirred fermentor, and metabolic products are withdrawn continuously at the same rate by crossflow microfiltration in an external recycle loop, so that product inhibition of cell growth is relieved. The maximum density of cells is increased fivefold more than in the control process, and is limited by acetic and other organic acids accumulating from the aerobic fermentation of excess glucose.

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Author notes

  1. Philipp Gerhardt: To whom correspondence should be directed.

Authors and Affiliations

  1. Department of Chemical Engineering, Michigan State University, East Lansing, MI, 48824

    Kevin W. Anderson & Eric Grulke

  2. Department of Microbiology and Public Health, Michigan State University, East Lansing, MI, 48824

    Philipp Gerhardt

Authors
  1. Kevin W. Anderson
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  2. Eric Grulke
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  3. Philipp Gerhardt
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Anderson, K., Grulke, E. & Gerhardt, P. Microfiltration Culture Process for Enhanced Production of rDNA Receptor Cells of Escherichia Coli. Nat Biotechnol 2, 891–896 (1984). https://doi.org/10.1038/nbt1084-891

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