Review Article | Published:

Plant cell cultures for the production of recombinant proteins

Nature Biotechnology volume 22, pages 14151422 (2004) | Download Citation

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Abstract

The use of whole plants for the synthesis of recombinant proteins has received a great deal of attention recently because of advantages in economy, scalability and safety compared with traditional microbial and mammalian production systems. However, production systems that use whole plants lack several of the intrinsic benefits of cultured cells, including the precise control over growth conditions, batch-to-batch product consistency, a high level of containment and the ability to produce recombinant proteins in compliance with good manufacturing practice. Plant cell cultures combine the merits of whole-plant systems with those of microbial and animal cell cultures, and already have an established track record for the production of valuable therapeutic secondary metabolites. Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems.

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Affiliations

  1. Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Worringerweg 1, D-52074 Aachen, Germany.

    • Stephan Hellwig
    • , Jürgen Drossard
    •  & Rainer Fischer
  2. Department of Biology, University of York, Heslington, York YO10 5DD, United Kingdom.

    • Richard M Twyman
  3. Institut für Biologie VII (Molekulare Biotechnologie), Aachen Technical University, D-52056 Aachen, Germany.

    • Rainer Fischer

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The authors declare no competing financial interests.

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Correspondence to Rainer Fischer.

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https://doi.org/10.1038/nbt1027

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