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Climbing the protein ladder

A simple hydrolysis-based approach to peptide analysis may facilitate high-throughput identification of post-translational modifications.

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Figure 1: Analysis of the N-terminal ladder of insulin by mass analysis of peptides (MAP).

Bob Crimi

References

  1. Zhong, H.Y., Zhang, Y., Wen, Z.H. & Li, L. Nat. Biotechnol. 22, XX–XX (2004).

    Article  Google Scholar 

  2. van Slyke, D.D. J. Biol. Chem. 10, 15–55 (1911).

    Google Scholar 

  3. Gordon, A.H., Martin, A.J.P. & Synge, R.L.M. Biochem. J. 35, 1369–1387 (1941).

    Article  CAS  Google Scholar 

  4. Sanger, F. Science 129, 1340–1344 (1959).

    Article  CAS  Google Scholar 

  5. Edman, P. Eur. J. Biochem. 1, 80–91 (1967).

    Article  CAS  Google Scholar 

  6. Tanaka, K. et al. Rapid Commun. Mass Spectrom. 2, 151–153 (1988).

    Article  CAS  Google Scholar 

  7. Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F. & Whitehouse, C.M. Science 246, 64–71 (1989).

    Article  CAS  Google Scholar 

  8. Consden, R., Gordon, A.H., Martin, A.J.P. & Synge, R.L.M. Biochem. J. 41, 596–602 (1947).

    Article  CAS  Google Scholar 

  9. Sanger, F., Nicklen, S. & Coulson, A.R. Proc. Natl. Acad. Sci. USA 74, 5463–5467 (1977).

    Article  CAS  Google Scholar 

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Dovichi, N. Climbing the protein ladder. Nat Biotechnol 22, 1242–1243 (2004). https://doi.org/10.1038/nbt1004-1242

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