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Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells


Since the advent of procedures for cloning animals, conservation biologists have proposed using this technology to preserve endangered mammals. Here we report the successful cloning of a wild endangered animal, Ovis orientalis musimon, using oocytes collected from a closely related, domesticated species, Ovis aries. We injected enucleated sheep oocytes with granulosa cells collected from two female mouflons found dead in the pasture. Blastocyst-stage cloned embryos transferred into sheep foster mothers established two pregnancies, one of which produced an apparently normal mouflon. Our findings support the use of cloning for the expansion of critically endangered populations.

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Figure 1: Cloned female mouflon with the sheep foster mother.
Figure 2


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Special thanks go to Dr. Cesare Galli (Laboratorio di Tecnologie della Riproduzione, Cremona, Italy) for constructive discussions throughout the experiments. The authors also acknowledge Dr. Michele Blasi for the microsatellite analysis, Dr. Maria Dattena and Mr. Fabrizio Chessa for help with surgery, and Mr. Giampiero Camoglio and Mr. Antonio Pintadu for animal care. J.F. is supported by GACR 524/96/K162.

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Correspondence to Pasqualino Loi.

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Loi, P., Ptak, G., Barboni, B. et al. Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat Biotechnol 19, 962–964 (2001).

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