Abstract
We have obtained transgenic plants from three cultivars of creeping bentgrass, a type of turfgrass used for golf greens. Two different vectors containing the bar gene, which confers resistance to the herbicide bialaphos, were used in the transformations. Transgenic plants were recovered from both plate and liquid selection of embryogenic suspension cultures on media containing 2 or 4 mg/l of bialaphos. Microprojectile bombardment of 8–14 filters in five different experiments, yielded a total of 271 plants that survived the initial spray concentration (1.5 mg/ml) of the commercial herbicide Herbiace™. Of these, 55 survived the higher spray rate (2 mg/ml). Presence of the bar gene in the bialaphos resistant plants was confirmed by PCR and Southern analysis. Transcripts corresponding to the bar gene were present in transgenic plants that showed bialaphos resistance. The 55 transgenic creeping bentgrass lines described are from the cultivars Emerald and Southshore. They are resistant to 5X the field rate of bialaphos and therefore may have commercial potential.
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References
Beard, J.B. Turfgrass: Science and Culture. 1973. Prentice-Hall, Inc., Engle-wood Cliffs, N. J. pp. 71–78.
Dahl, A.S. 1934. The relation between rainfall and injuries to turf-season 1933. Greenskeepers' Reporter 2(2): 1–4.
Cheesman, J.H. and Roberts, E.C. 1964. Effect of nitrogen level and moisture stress on Helminthosporium infection in Merion bluegrass. 1964 Agronomy Abstracts: 105.
Jefferson, R.N. and Morishita, J.S. 1962. Progress report on the bermuda grass mite and the fruit fly. California Turfgrass Culture 12(2): 9–10.
Krans, J.V., Henning, V.T. and Torres, K.C. 1982. Callus induction, maintenance and plantlet regeneration in creeping bentgrass. Crop Science 22: 1193–1197.
Blanche, F.C., Krans, J.V. and Coats, G.E. 1986. Improvement in callus growth and plantlet formation in creeping bentgrass. Crop Science 26: 1245–1248.
Zhong, H., Srinivasan, C. and Sticklen, B. 1991. Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustris Huds). Plant Cell Reports 10: 453–456.
Terakawa, T., Sato, T. and Koike, M. 1992. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Reports 11: 457–461.
Zhong, H., Bolyard, M.G., Srinivasan, C. and Sticklen, M.B. 1993. Transgenie plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus. Plant Cell Reports 13: 1–6.
Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J. and Klein, T.M. 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–839.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.
Potrykus, I., Saul, M.W., Petruska, J., Paszkowski, J. and Shillito, R. 1985. Direct gene transfer to cells of a graminaceous monocot. Mol. Gen. Genet. 199: 183–188.
Hauptmann, R.M., Vasil, V., Ozias-Atkins, P., Tabbaeiizadeh, Z., Rogers, S.G., Fraley, R.T., Horsch, R.B. and Vasil, I.K. 1988. Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol. 86: 602–606.
Horn, M.E., Shillito, R.D., Conger, B.V. and Harms, C.T. 1988. Transgenic plants of Orchardgrass (Dactylis glomerata L.) from protoplasts. Plant Cell Reports 7: 469–472.
Ha, S.-B., Wu, F.-S. and Thome, T.K. 1992. Transgenic turf-type tall fescue (Festuca arundinacea Screb. ) plants regenerated from protoplasts. Plant Cell Reports 11: 601–604.
Wang, Z., Takamizo, T., Iglesias, V.A., Osusky, M., Nagel, J., Potrykus, I. and Spangenberg, G. 1992. Transgenic plants of tall fescue (Festua arundinacea Screb.) obtained by direct gene transfer to protoplasts. Bio/Technology 10: 691–696.
Balogh, J.C. and Walker, W.J. 1992. Golf course management and construction environmental issues. Lewis Publishers, Boca Raton, FL.
Kross, B.C., Burmeister, L.F. and Ogilivie, L.K. 1994. Mortality study among golf course superintendents. Golf Course Management 62: 49–56.
Sambrook, J., Fritsch, E.F. and Maniatis 1989. Molecular Cloning: a Laboratory Manual, Second edition. Cold Spring Harbor laboratory Press, N. Y.
Tumer, N.E., Clarke, W.G., Tabor, G.J., Hironaka, C.M., Fraley, R.T. and Shah, D.M. 1986. The genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase are expressed differentially in petunia leaves. Nuc. Acids Res. 14: 3325–3342.
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Hartman, C., Lee, L., Day, P. et al. Herbicide Resistant Turfgrass (Agrostis palustris Huds.) by Biolistic Transformation. Nat Biotechnol 12, 919–923 (1994). https://doi.org/10.1038/nbt0994-919
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DOI: https://doi.org/10.1038/nbt0994-919
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