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Gene Transfer to Tumor–Infiltrating Lymphocytes and other Mammalian Somatic Cells by Microprojectile Bombardment

Bio/Technology volume 10, pages 1036–1040 (1992)Cite this article

Abstract

We demonstrate the application of particle delivery to the transformation of mammalian somatic cells. Two mouse T–lymphocyte cell lines and Chinese hamster ovary (CHO) cells were transformed transiently with the RSV–ADH (alcohol dehydrogenase) gene. Stable gene transfer was also demonstrated in CHO cells at a frequency of 6 × 10−4 and in T–cells at a frequency of 1 × 10−4, using β–Gal/neomycin–resistance gene constructs. The helium gas acceleration mechanism (PDS 1000/He) and particle delivery method allowed better velocity control and particle dispersion than the gunpowder driven instrument. These improvements have increased cell viability, transformation efficiency, and cellular targets.

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  1. DCV DuPont/ConAgra, 3411 Silverside Rd., Concord Plaza/Quillen Wilmington, DE, 19810

    Sandra Fitzpatrick-McElligott

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Fitzpatrick-McElligott, S. Gene Transfer to Tumor–Infiltrating Lymphocytes and other Mammalian Somatic Cells by Microprojectile Bombardment. Nat Biotechnol 10, 1036–1040 (1992). https://doi.org/10.1038/nbt0992-1036

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