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Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.)

Bio/Technology volume 9, pages 864–868 (1991)Cite this article

Abstract

We have recovered transgenic carnation plants on kanamycin-containing medium after co-cultivation of stems with disarmed or armed strains of Agrobacterium tumefaciens. Both strains contained a vector carrying the neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) genes. Optimal conditions for transformation and patterns of GUS expression in transgenic plants are described. Over two hundred transgenic plants have been established in soil.

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References

  1. The International Floriculture Quarterly Report. October 1990. Pert-wee, J (Ed.). Pathfast Publications, U.K.

  2. Mol, J.N.M., Stuitje, A.R., Gerats, A., Van der Krol, A. and Jorgensen, R. 1989. Saying it with genes: Molecular flower breeding. Tibtech 7: 148–153.

    Article  CAS  Google Scholar 

  3. Mol, J.N.M., Stuitje, A.R. and Van der Krol, A. 1989. Genetic manipulation of floral pigmentation genes. Plant Mol. Biol. 13: 287–294.

    Article  CAS  PubMed  Google Scholar 

  4. von Wettstein, D. 1989. Perspectives for the genetic engineering of plants for agriculture, horticulture and industry. Plant Mol. Biol. 13: 313–317.

    Article  CAS  PubMed  Google Scholar 

  5. Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240: 204–207.

    Article  CAS  PubMed  Google Scholar 

  6. Shimamoto, K., Terada, R., Isawa, T. and Fujimoto, H. 1989. Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–276.

    Article  CAS  Google Scholar 

  7. Fry, J., Barnason, A. and Horsch, R.B. 1987. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors. Plant Cell Rep. 6: 321–325.

    Article  CAS  PubMed  Google Scholar 

  8. McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R. and Fraley, R. 1986. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Rep. 5: 81–84.

    Article  CAS  PubMed  Google Scholar 

  9. De Block, M. 1988. Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens. Theor. Appl. Genet. 76: 767–774.

    Article  CAS  PubMed  Google Scholar 

  10. James, J.D., Passey, A.J., Barbara, D.J. and Bevan, M. 1989. Genetic transformation of apple (Malus pumila Mill) using a disarmed Ti-binary vector. Plant Cell Rep. 7: 658–661.

    CAS  PubMed  Google Scholar 

  11. Van der Krol, A., Lenting, P.E., Veenstra, J., Van der Meer, I.M., Koes, R.E., Gerats, A.G.M., Mol, J.N.M. and Stuitje, A.R. 1988. An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333: 866–869.

    Article  CAS  Google Scholar 

  12. Van der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N.M. and Stuitje, A.R. 1990. Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. The Plant Cell 2: 291–299.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Smith, C.J.S., Watson, C.F., Ray, J., Bird, C.R., Morris, P.C., Schuch, W. and Grierson, D. 1988. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334: 724–726.

    Article  CAS  Google Scholar 

  14. Cornish, E. and Stevenson, T. 1990. Designer flowers: a blossoming industry. Todays Life Science January: 46–47.

    Google Scholar 

  15. Nelson, R.S., McCormick, S.M., Delannay, X., Dube, P., Layton, J., Anderson, E.J., Kaniewska, M., Proksch, R.K., Horsch, R.B., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1988. Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.

    Google Scholar 

  16. Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., De Beuckeleer, M., Dean, C., Zabeau, M., Van Montagu, M. and Leemans, J. 1987. Transgenic plants protected from insect attack. Nature 328: 33–37.

    Article  CAS  Google Scholar 

  17. Lu, C., Nugent, G. and Wardley, T. 1990. Efficient, direct plant regeneration form stem segments of Chrysanthemum morifolium Ramat. cv. Royal Purple. Plant Cell Rep. 8: 733–736.

    Article  CAS  PubMed  Google Scholar 

  18. Chabaud, M., Passiatore, J.E., Cannon, F. and Buchanan-Wollaston, V. 1988. Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformation. Plant Cell Rep. 7: 512–516.

    Article  CAS  PubMed  Google Scholar 

  19. Jia, S.-R., Yang, M.-Z., Ott, R. and Chua, N.-H. 1989. High frequency transformation of Kalanchoe laciniata. Plant Cell Rep. 8: 336–340.

    Article  CAS  PubMed  Google Scholar 

  20. Binns, A.N. and Thomashow, M.F. 1988. Cell biology of Agrobacterium infection and transformation of plants. Ann. Rev. Microbiol. 42: 575–606.

    Article  CAS  Google Scholar 

  21. Chapel, M. and Glimelius, K. 1990. Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts. Plant Cell Rep. 9: 105–108.

    Article  CAS  PubMed  Google Scholar 

  22. Williamson, J.D., Hirsh-Wyncott, M.E., Larkins, B.A. and Gelvin, S.B. 1989. Differential accumulation of a transcript driven by the CaMV 35S promoter in transgenic tobacco. Plant Physiol. 90: 1570–1576.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Benfey, P.N., Ren, L. and Chua, N.H. 1989. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J. 8: 2195–2202.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Smigocki, A.C. and Owens, L.D. 1988. Cytokinin gene fused with a strong promoter enhances shoot organogenesis and zeatin levels in transformed plant cells. Proc. Natl. Acad. Sci. USA 85: 5131–5135.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Janssen, B.J. and Gardner, R.C. 1989. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium. Plant Mol. Biol. 14: 61–72.

    Article  Google Scholar 

  27. Garfinkel, D.J. and Nester, E.W. 1980. Agrobacterium tumefaciens mutants affected in crown gall tumorigenisis and octopine catabolism. J. Bact. 144: 732–743.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 73–97.

    Article  Google Scholar 

  29. Kandreck, K.A. and Black, N.D. 1984. Growing Media for Ornamental Plants and Turf, p. 317. NSW University Press, Kensington, NSW, Australia.

    Google Scholar 

  30. Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol Rep. 5: 387–405.

    Article  CAS  Google Scholar 

  31. McDonnell, R.E., Clark, R.D., Smith, W.A. and Hinchee, M.A. 1987. A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues. Plant Mol. Biol. Rep. 4: 380–386.

    Article  Google Scholar 

  32. Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1: 19–21.

    Article  CAS  Google Scholar 

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Author notes

  1. Terese Wardley-Richardson

    Present address: Division of Plant Industry CSIRO, GPO Box 1600, Canberra, ACT, 2601, Australia

  2. Chin-Yi Lu: Corresponding author.

Authors and Affiliations

  1. Calgene Pacific Pty Ltd, 16 Gipps Street, Collingwood, Victoria, 3066, Australia

    Chin-Yi Lu, Greg Nugent, Stephen F. Chandler, Richard Young & Michael J. Dalling

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  1. Chin-Yi Lu
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  2. Greg Nugent
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  6. Michael J. Dalling
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Lu, CY., Nugent, G., Wardley-Richardson, T. et al. Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.). Nat Biotechnol 9, 864–868 (1991). https://doi.org/10.1038/nbt0991-864

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  • DOI: https://doi.org/10.1038/nbt0991-864

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