Research Paper | Published:

Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.)

Abstract

We have recovered transgenic carnation plants on kanamycin-containing medium after co-cultivation of stems with disarmed or armed strains of Agrobacterium tumefaciens. Both strains contained a vector carrying the neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) genes. Optimal conditions for transformation and patterns of GUS expression in transgenic plants are described. Over two hundred transgenic plants have been established in soil.

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References

  1. 1

    The International Floriculture Quarterly Report. October 1990. Pert-wee, J (Ed.). Pathfast Publications, U.K.

  2. 2

    Mol, J.N.M., Stuitje, A.R., Gerats, A., Van der Krol, A. and Jorgensen, R. 1989. Saying it with genes: Molecular flower breeding. Tibtech 7: 148–153.

  3. 3

    Mol, J.N.M., Stuitje, A.R. and Van der Krol, A. 1989. Genetic manipulation of floral pigmentation genes. Plant Mol. Biol. 13: 287–294.

  4. 4

    von Wettstein, D. 1989. Perspectives for the genetic engineering of plants for agriculture, horticulture and industry. Plant Mol. Biol. 13: 313–317.

  5. 5

    Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240: 204–207.

  6. 6

    Shimamoto, K., Terada, R., Isawa, T. and Fujimoto, H. 1989. Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–276.

  7. 7

    Fry, J., Barnason, A. and Horsch, R.B. 1987. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors. Plant Cell Rep. 6: 321–325.

  8. 8

    McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R. and Fraley, R. 1986. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Rep. 5: 81–84.

  9. 9

    De Block, M. 1988. Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens. Theor. Appl. Genet. 76: 767–774.

  10. 10

    James, J.D., Passey, A.J., Barbara, D.J. and Bevan, M. 1989. Genetic transformation of apple (Malus pumila Mill) using a disarmed Ti-binary vector. Plant Cell Rep. 7: 658–661.

  11. 11

    Van der Krol, A., Lenting, P.E., Veenstra, J., Van der Meer, I.M., Koes, R.E., Gerats, A.G.M., Mol, J.N.M. and Stuitje, A.R. 1988. An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333: 866–869.

  12. 12

    Van der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N.M. and Stuitje, A.R. 1990. Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. The Plant Cell 2: 291–299.

  13. 13

    Smith, C.J.S., Watson, C.F., Ray, J., Bird, C.R., Morris, P.C., Schuch, W. and Grierson, D. 1988. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334: 724–726.

  14. 14

    Cornish, E. and Stevenson, T. 1990. Designer flowers: a blossoming industry. Todays Life Science January: 46–47.

  15. 15

    Nelson, R.S., McCormick, S.M., Delannay, X., Dube, P., Layton, J., Anderson, E.J., Kaniewska, M., Proksch, R.K., Horsch, R.B., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1988. Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.

  16. 16

    Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., De Beuckeleer, M., Dean, C., Zabeau, M., Van Montagu, M. and Leemans, J. 1987. Transgenic plants protected from insect attack. Nature 328: 33–37.

  17. 17

    Lu, C., Nugent, G. and Wardley, T. 1990. Efficient, direct plant regeneration form stem segments of Chrysanthemum morifolium Ramat. cv. Royal Purple. Plant Cell Rep. 8: 733–736.

  18. 18

    Chabaud, M., Passiatore, J.E., Cannon, F. and Buchanan-Wollaston, V. 1988. Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformation. Plant Cell Rep. 7: 512–516.

  19. 19

    Jia, S.-R., Yang, M.-Z., Ott, R. and Chua, N.-H. 1989. High frequency transformation of Kalanchoe laciniata. Plant Cell Rep. 8: 336–340.

  20. 20

    Binns, A.N. and Thomashow, M.F. 1988. Cell biology of Agrobacterium infection and transformation of plants. Ann. Rev. Microbiol. 42: 575–606.

  21. 21

    Chapel, M. and Glimelius, K. 1990. Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts. Plant Cell Rep. 9: 105–108.

  22. 22

    Williamson, J.D., Hirsh-Wyncott, M.E., Larkins, B.A. and Gelvin, S.B. 1989. Differential accumulation of a transcript driven by the CaMV 35S promoter in transgenic tobacco. Plant Physiol. 90: 1570–1576.

  23. 23

    Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907.

  24. 24

    Benfey, P.N., Ren, L. and Chua, N.H. 1989. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J. 8: 2195–2202.

  25. 25

    Smigocki, A.C. and Owens, L.D. 1988. Cytokinin gene fused with a strong promoter enhances shoot organogenesis and zeatin levels in transformed plant cells. Proc. Natl. Acad. Sci. USA 85: 5131–5135.

  26. 26

    Janssen, B.J. and Gardner, R.C. 1989. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium. Plant Mol. Biol. 14: 61–72.

  27. 27

    Garfinkel, D.J. and Nester, E.W. 1980. Agrobacterium tumefaciens mutants affected in crown gall tumorigenisis and octopine catabolism. J. Bact. 144: 732–743.

  28. 28

    Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 73–97.

  29. 29

    Kandreck, K.A. and Black, N.D. 1984. Growing Media for Ornamental Plants and Turf, p. 317. NSW University Press, Kensington, NSW, Australia.

  30. 30

    Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol Rep. 5: 387–405.

  31. 31

    McDonnell, R.E., Clark, R.D., Smith, W.A. and Hinchee, M.A. 1987. A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues. Plant Mol. Biol. Rep. 4: 380–386.

  32. 32

    Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1: 19–21.

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