Research Paper | Published:

Direct Somatic Embryogenesis in Peanut (Arachis Hypogea)

Bio/Technology volume 7, pages 949951 (1989) | Download Citation

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Abstract

We have regenerated plants of Arachis hypogea cultivar SB-11 from immature zygotic embryo axis via direct somatic embryogenesis without an intermediate callus stage. Induction and maturation of the somatic embryos was achieved on the same medium. 2,4-dichlorophenoxy acetic acid (2,4-D) was essential for this response. Both the size of the immature zygotic embryo axis and the concentration of 2,4-D were important for direct somatic embryogenesis. Embryos germinated in hormone free medium, and plants survived in a sand:soil mixture.

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Author notes

    • A. F. Mascarenhas

    Corresponding author.

Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, Poona—411 008, India.

    • S. Hazra
    • , S. S. Sathaye
    •  & A. F. Mascarenhas

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DOI

https://doi.org/10.1038/nbt0989-949

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