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Expression of Human β1 and β2 Adrenergic Receptors in E. Coli As a New Tool for Ligand Screening

Bio/Technologyvolume 7pages923927 (1989) | Download Citation

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Abstract

E. coli transformed with appropriate plasmid vectors express human β1 and β2-adrenergic receptors that retain the binding properties of the corresponding receptors in mammalian tissues. The experimental procedure required for pharmacological studies on active receptors expressed in bacteria is considerably simplified compared to that used for the same experiments in reference tissues. Moreover, in this bacterial system, receptor-binding assays display low background and high reproducibility. These advantages demonstrate that bacterial clones expressing membrane receptors constitute an alternative screening procedure for synthetic compounds specific for each β-adrenergic receptor subtype.

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Author information

Affiliations

  1. Laboratoire de Biologie Moléculaire des Récepteurs, Institut Pasteur, 25 Rue du Docteur Roux, 75724, Paris, Cedex 15, France

    • Stefano Marullo
    • , Colette Delavier-Klutchko
    • , Jean-Gérard Guillet
    • , Arthur Donny Strosberg
    •  & Laurent Jean Emorine
  2. Unité de Programmation Moléculaire et Toxicologie Génétique, Institut Pasteur, 25 Rue du Docteur Roux, 75724, Paris, Cedex 15, France

    • Alain Charbit

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https://doi.org/10.1038/nbt0989-923

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