Research Paper | Published:

Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis

Bio/Technologyvolume 2pages808811 (1984) | Download Citation



The multi-copy plasmid vector pIJ702 has been used to clone Streptomyces clavuligerus chromosomal DNA fragments into a clavulanic acid non-producing mutant strain. Two DNA segments, which restored the ability to synthesise clavulanic acid, were shown to overlap one another and share a common sequence of about 2 kb. A preliminary restriction map of the region of the chromosome involved has been constructed.

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  1. Christopher R. Bailey and David J. Winstanley: Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey, RH3 7AJ, England

  2. Michael J. Butler: Pfizer Limited, Ramsgate Road, Sandwich, Kent, CT13 9NJ, England

  3. Robert T. Rowlands: Panlab Genetics, Inc., 110-110th Avenue N.E., Suite 704, Bellevue, WA 98004, USA


  1. Beecham Pharmaceuticals UK Division, Clarendon Road, Worthing, West Sussex, BN14 8QH, England

    • Ian D. Normansell


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