Research Paper | Published:

Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis

Bio/Technology volume 2, pages 808811 (1984) | Download Citation

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Abstract

The multi-copy plasmid vector pIJ702 has been used to clone Streptomyces clavuligerus chromosomal DNA fragments into a clavulanic acid non-producing mutant strain. Two DNA segments, which restored the ability to synthesise clavulanic acid, were shown to overlap one another and share a common sequence of about 2 kb. A preliminary restriction map of the region of the chromosome involved has been constructed.

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Author information

Author notes

    • Christopher R. Bailey
    •  & David J. Winstanley

    Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey, RH3 7AJ, England

    • Michael J. Butler

    Pfizer Limited, Ramsgate Road, Sandwich, Kent, CT13 9NJ, England

    • Robert T. Rowlands

    Panlab Genetics, Inc., 110-110th Avenue N.E., Suite 704, Bellevue, WA 98004, USA

Affiliations

  1. Beecham Pharmaceuticals UK Division, Clarendon Road, Worthing, West Sussex, BN14 8QH, England

    • Ian D. Normansell

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DOI

https://doi.org/10.1038/nbt0984-808

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