Research Paper | Published:

Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis

Bio/Technology volume 2, pages 808811 (1984) | Download Citation



The multi-copy plasmid vector pIJ702 has been used to clone Streptomyces clavuligerus chromosomal DNA fragments into a clavulanic acid non-producing mutant strain. Two DNA segments, which restored the ability to synthesise clavulanic acid, were shown to overlap one another and share a common sequence of about 2 kb. A preliminary restriction map of the region of the chromosome involved has been constructed.

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  1. 1.

    and 1977. Clavulanic acid: A beta-lactamase-inhibiting beta-lactam From Streptomyces clavuligerus . Antimicrob. Agents Chemother. 11: 852–857.

  2. 2.

    , , and 1982. Gene cloning in Streptomyces . Curr. Top. Microbiol. Immunol. 96: 69–95.

  3. 3.

    and 1982. Cloning in Streptomyces: Systems and strategies, p. 119–145. In: Genetic Engineering, Setlow, J.K. and Hollaender, A. (eds.), vol. 4, Plenum Press, New York.

  4. 4.

    , , and 1983. Cloning arid expression of the tyrosinase geric from Streptomyces antibioticus in Streptomyces lividans . J. Gen. Microbiol. 129: 2703–2714.

  5. 5.

    , , and 1982. Cloning of antibiotic resistance and nutritional genes in Streptomyces . J. Bacteriol. 151: 668–677.

  6. 6.

    , , and 1982. Spectrophotometric assay of clavulamt acid by reaction with imidazole. Analyst 107: 1241–1245.

  7. 7.

    and 1982. Assay of amoxicillin and clavulanic acid, the components of Augmentin, in biological fluids with high-performance liquid chromatography. Antimicrob. Agents Chemother. 22: 753–762.

  8. 8.

    1983. Actinomycete genetics and antibiotic production, p 1–24. In: Biochemistry and Genetic Regulation of Commercially Important Antibiotics. Vining, L.C. (ed.), Addison-Wesley, Reading, MA.

  9. 9.

    , , , , , and 1983. Cloning Streptomyces genes for antibiotic production. Trends in Biotechnology 1: 42–48.

  10. 10.

    and 1984. Cloning and expression of antibiotic production genes. Biotechnology 2: 63–72.

  11. 11.

    and 1983. Cloning of a Streptomyces gene for an o-methyltransfcrase involved in antibiotic biosynthesis. Molec. Gen. Genet. 190: 394–398.

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    • Christopher R. Bailey
    •  & David J. Winstanley

    Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey, RH3 7AJ, England

    • Michael J. Butler

    Pfizer Limited, Ramsgate Road, Sandwich, Kent, CT13 9NJ, England

    • Robert T. Rowlands

    Panlab Genetics, Inc., 110-110th Avenue N.E., Suite 704, Bellevue, WA 98004, USA


  1. Beecham Pharmaceuticals UK Division, Clarendon Road, Worthing, West Sussex, BN14 8QH, England

    • Ian D. Normansell


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