Newer Methods for Characterization of Antibiotics II. Antibiotic Structure Studied by Mass Spectrometry


The use of FAB and FD as well as EI mass spectrometry for the determination of antibiotic structures is described. Neomycins, scopafungin, polyenic macrolides, alamethicins, emerimicins, and antiamoebins proved to be amenable to FD analysis while the capability of FAB is demonstrated with zwitterionic tetraenes, and zervamicins. Due to the well characterized fragmentations it produces, EI is used whenever the sample or its degradation products are sufficiently volatile.

*E. R. Squibb lectures on Chemistry of Microbial Products presented at Waksman Institute of Microbiology, Rutgers University, New Brunswick, NJ, 16, March 1983.

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  1. 1

    Waller, G.R., and Dermer, O.C. (eds). 1980 Biochemical Applications of Mass Spectrometry, First Supplementary Volume. Wiley-Interscience, New York.

  2. 2

    Macfarlane, R.D. 1980. pp. 1209–1218. 252Cf-plasma desorption mass spectrometry (PDMS). In G.R. Waller and O.C. Dermer (eds), Biochemical Applications of Mass Spectrometry, First Supplementary Volume, Wiley-Interscience, New York.

  3. 3

    Beckey, H.D. 1977. Principles of field ionization and field desorption mass spectrometry. Pergamon, Oxford.

  4. 4

    Olson, K.L., Cook, J.C., Jr., and Rinehart, K.L., Jr., 1974. A quantitative method of sample loading for field desorption mass spectrometry. Biomed. Mass Spectrom. 1: 358–362.

  5. 5

    Rinehart, K.L., Jr., 1982. Fast atom bombardment mass spectrometry. Science 218: 254–260.

  6. 6

    Rinehart, K.L., Jr., Cook, J.C., Jr., Pandey, R.C., Gaudioso, L.A., Meng, H., Moore, M.L., Gloer, J. B., Wilson, G.R., Gutowsky, R.E., Zierath, P.D., Shield, L.S., Li, H.L., Renis, H.E., McGovren, J.P., and Canonico, P.G. 1982. Biologically active peptides and their mass spectra. Pure Appl. Chem. 54: 2409–2424.

  7. 7

    Rinehart, K.L., Jr., 1977. Mutasynthesis of new antibiotics. Pure Appl. Chem. 49: 1361–1384.

  8. 8

    Samain, D., Cook, J.C., Jr., and Rinehart, K. L., Jr., 1982. The structure of scopafungin, a potent nonpolyene antifungal antibiotic. J. Am. Chem. Soc. 104: 4129–4141.

  9. 9

    Rinehart, K.L., Jr., Cook, J.C., Jr., Maurer, K. H. and Rapp, U. 1974. Field desorption mass spectra of antibiotics. J. Antibiot. 27 1–13.

  10. 10

    Pandey, R.C., Rinehart, K.L., Jr., and Millington, D.S. 1980. The structures of dermostatins A and B. Hind. Antibiot. Bull. 22: 47–61.

  11. 11

    Pandey, R.C. and Rinehart, K.L., Jr., 1976. Polyene antibiotics. VII. Carbon-13 nuclear magnetic resonance evidence for cyclic hemiketals in the polyene antibiotics amphotericin B, nystatin A1, tetrin A, tetrin B, lucensomycin, and pimaricin. J. Antibiot. 29: 1035–1042.

  12. 12

    Loewenstein, W.R. 1978. Membrane channels. Introductory remarks. Fed. Proc. 37: 2626–2627, and following papers.

  13. 13

    Rinehart, K.L., Jr., Gaudioso, L.A., Moore, M.L., Pandey, R.C., Cook, J.C., Jr., Barber, M., Sedgwick, R.D., Bordoli, R. S., Tyler, A.N. and Green, B.N. 1981. Structures of eleven zervamicin and two emerimicin peptide antibiotics studied by fast atom bombardment mass spectrometry. J. Am. Chem. Soc. 103: 6517–6520.

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Rinehart, K. Newer Methods for Characterization of Antibiotics II. Antibiotic Structure Studied by Mass Spectrometry. Nat Biotechnol 1, 581–588 (1983) doi:10.1038/nbt0983-581

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