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Agrobacterium-mediated DNA transfer, and then some

Nature Biotechnology volume 26, pages 998–1000 (2008)Cite this article

In addition to its plasmid DNA, Agrobacterium tumefaciens can transfer its chromosomal DNA to plant genomes.

The emergence of today's agricultural biotechnology industry stems largely from the discovery, ∼30 years ago, that the pathogenic soil bacterium Agrobacterium tumefaciens can integrate so-called transfer DNA (T-DNA) on the tumor-inducing (Ti) plasmid into the genomes of most crops. In the late 1990s, scientists found that the transferred sequences can include regions of the Ti plasmid, or T-DNA 'binary vectors', outside the T-DNA borders1. In this issue, Ülker et al.2 show that the plant genome may also incorporate bacterial chromosomal DNA.

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Figure 1: Models for various mechanisms to link T-DNA to Agrobacterium chromosomal DNA.

References

  1. Kononov, M.E., Bassuner, B. & Gelvin, S.B. Plant J. 11, 945–957 (1997).

    CAS  Article  Google Scholar 

  2. Ülker, B. et al. Nat. Biotechnol. 26, 1015–1017 (2008).

    Article  Google Scholar 

  3. Wood, D.W. et al. Science 294, 2317–2323 (2001).

    CAS  Article  Google Scholar 

  4. Goodner, B. et al. Science 294, 2323–2328 (2001).

    CAS  Article  Google Scholar 

  5. Furner, I.J. et al. Nature 319, 422–427 (1986).

    CAS  Article  Google Scholar 

  6. Suzuki, K., Yamashita, I. & Tanaka, N. Plant J. 32, 775–787 (2002).

    CAS  Article  Google Scholar 

  7. Meyer, A.D., Ichikawa, T. & Meins, F. Mol. Gen. Genet. 249, 265–273 (1995).

    CAS  Article  Google Scholar 

  8. Ichikawa, T., Ozeki, Y. & Syono, K. Mol. Gen. Genet. 220, 177–180 (1990).

    CAS  Article  Google Scholar 

  9. Durrenberger, F., Crameri, A., Hohn, B. & Koukolikova-Nicola, Z. Proc. Natl. Acad. Sci. USA 86, 9154–9158 (1989).

    CAS  Article  Google Scholar 

  10. Rommens, C.M. et al. Plant Physiol. 139, 1338–1349 (2005).

    CAS  Article  Google Scholar 

  11. De Neve, M. et al. Plant J. 11, 15–29 (1997).

    CAS  Article  Google Scholar 

Download references

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  1. Stanton B. Gelvin is in the Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA. gelvin@bilbo.bio.purdue.edu

    Stanton B Gelvin

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  1. Stanton B Gelvin
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Gelvin, S. Agrobacterium-mediated DNA transfer, and then some. Nat Biotechnol 26, 998–1000 (2008). https://doi.org/10.1038/nbt0908-998

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  • DOI: https://doi.org/10.1038/nbt0908-998

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