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On the iTRAQ of kinase inhibitors

Cellular targets of kinase inhibitors are identified by mass spectrometric analysis of binding to “kinobeads.”

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Figure 1: Cells or cell lysates are incubated with increasing concentrations of a given kinase inhibitor (such as imatinib) and kinases are isolated by affinity enrichment with kinobeads displaying broad-specificity kinase inhibitors.

Kim Caesar


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White, F. On the iTRAQ of kinase inhibitors. Nat Biotechnol 25, 994–996 (2007).

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