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Keeping dry and crossing membranes

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Figure 1: Wrapping of the glucagon molecule in helical conformation (PDB entry 1GCN).
Figure 2: Time dependence of the adsorption uptake and desorption of soluble mutants of the G4V glucagon peptide onto and from a dilauryl phosphatidylcholine bilayer coating a biosensing optical device.

Bob Crimi


  1. Fernández, A. & Scheraga, H.A. Proc. Natl. Acad. Sci. USA 100, 113–118 (2003).

    Article  Google Scholar 

  2. Fernández, A. & Berry, R.S. Proc. Natl. Acad. Sci. USA 100, 2391–2396 (2003).

    Article  Google Scholar 

  3. Fernández, A., Kardos, J., Scott, R.L., Goto, Y. & Berry, R.S. Proc. Natl. Acad. Sci. USA 100, 6446–6451 (2003).

    Article  Google Scholar 

  4. Fernández, A., Scott, R.L. & Berry, R.S. Proc. Natl. Acad. Sci. USA 101, 2823–2827 (2004).

    Article  Google Scholar 

  5. Fernández, A., Sosnick, T.R. & Colubri, A.J. Mol. Biol. 321, 659–675 (2002).

    Article  Google Scholar 

  6. Fändrich, M. & Dobson, C.M. EMBO J. 21, 5682–5690 (2002).

    Article  Google Scholar 

  7. Fernández, A. & Scott, R.L. Phys. Rev. Lett. 91, 18102–18106 (2003).

    Article  Google Scholar 

  8. Ball, P. Nature 423, 25–26 (2003).

    Article  CAS  Google Scholar 

  9. Lum, K., Chandler, D. & Weeks, J.D. J. Phys. Chem. B 103, 4570–4577 (1999).

    Article  CAS  Google Scholar 

  10. Hummer, G., Rasaiah, J.C. & Noworyta, J.P. Nature 414, 188–190 (2002).

    Article  Google Scholar 

  11. Fernández, A. J. Chem. Phys. 119, 5315–5319 (2003).

    Article  Google Scholar 

  12. Roseman, M.A. J. Mol. Biol. 201, 621–624 (1988).

    Article  CAS  Google Scholar 

  13. Fernández, A. & Scott, R.L. Biophys. J. 85, 1914–1928 (2003).

    Article  Google Scholar 

  14. Carey, C., Cheng, Y.-K. & Rossky, P. Chem. Phys. 258, 415–425 (2000).

    Article  CAS  Google Scholar 

  15. Goldberg, M. & Gomez-Orellana, I. Nat. Rev. Drug Discov. 2, 289–295 (2003).

    Article  CAS  Google Scholar 

  16. Leone-Bay, A. et al. Pharm. Res. 18, 964–970 (2001).

    Article  CAS  Google Scholar 

  17. Unson, C.G. & Merrifield, R.B. Proc. Natl. Acad. Sci. USA 91, 454–458 (1994).

    Article  CAS  Google Scholar 

  18. Dobbs, R. et al. Science 187, 544–547 (1975).

    Article  CAS  Google Scholar 

  19. Sapse, A.M., Rothchild, R., Jain, D.C. & Unson, C.G. Mol. Med. 8, 251–262 (2002).

    Article  CAS  Google Scholar 

  20. Fernández, A., Scott, R.L. & Scheraga, H.A. J. Phys. Chem. B 107, 9929–9932 (2003).

    Article  Google Scholar 

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This research was supported in part by an unrestricted grant from Eli Lilly and Company. The work was motivated by conversations with Richard DiMarchi.

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Fernández, A. Keeping dry and crossing membranes. Nat Biotechnol 22, 1081–1084 (2004).

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