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In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Nature Biotechnology volume 14, pages 992–994 (1996)Cite this article

Abstract

Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N-(dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

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Author notes

  1. Tetsuhiko Yoshimura: e-mail: yoshi@ymgt-techno.or.jp.

Authors and Affiliations

  1. Institute for Life Support Technology, Yamagata Technopolis Foundation, 2-2-1 Matsuei, Yamagata, 990, Japan

    Tetsuhiko Yoshimura, Hidekatsu Yokoyama, Satoshi Fujii & Hitoshi Kamada

  2. Department ofPharmacology, Oita Medical University, 1-1, Idaigaoka, Hasama-machi, Oita, 879-55, Japan

    Fusako Takayama

  3. Yamagata Research Institute of Technology, 2-2-1 Matsuei, Yamagata, 990, Japan

    Kazuo Oikawa

Authors
  1. Tetsuhiko Yoshimura
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  2. Hidekatsu Yokoyama
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  3. Satoshi Fujii
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  4. Fusako Takayama
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  5. Kazuo Oikawa
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  6. Hitoshi Kamada
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Yoshimura, T., Yokoyama, H., Fujii, S. et al. In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice. Nat Biotechnol 14, 992–994 (1996). https://doi.org/10.1038/nbt0896-992

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