Abstract
Toxins have been shown to be an important virulence component for most pathovars of Pseudomonas syringae. Here we have examined the role of phaseolotoxin in the virulence mechanism of P. syringae pv. phaseolicola by producing transgenic tobacco plants that express a pathogen–derived toxin–resistant target enzyme. Such plants are insensitive to the toxin and less prone to infection by the pathogen.
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References
Mitchell, R.E. 1984. The relevance of non-host-specific toxins in the expression of virulence by pathogens. Ann. Rev. Phytopathol. 22: 215–245.
Mitchell, R.E. 1976. Isolation and structure of a chlorosis-inducing toxin of Pseudomonas phaseolicola. Phytochemistry 15: 1941–1947.
Moore, R.E., Niemczura, W.P., Kwok, O.C.H. and Patil, S.S. 1984. Inhibitions of ornithine carbamoyltransferase from Pseudomonas syringae pv. phaseolicola. Revised structure of phaseolotoxin. Tetrahedron Lett. 25: 3931–3934.
Panopoulos, N.J., Lindgren, P.B., Peet, R.C., Thom, R.F., Hickman, M., Gies, D.R. and Willis, D.K. 1985. Molecular analysis of pathogenicity and virulence in Psfudomonas syringae pathovars, p. 188–192. In: Advances in Molecular Genetics of the Bacteria-Plant Interaction. Szalay, A. A and R. P. Legocki (Eds.). Cornell University Press, Ithaca, NY.
Patil, S.S., Kolattukudy, P.E. and Dimond, A.E. 1970. Inhibition of ornithine carbamoyltransferase from bean plants by the toxin of Pseudomonas phaseolicola. Plant Physiol. 46: 752–753.
Shargool, P.D., Jain, J.C. and McKay, G. 1988. Ornithine biosynthesis, and arginine biosynthesis and degradation in plant cells. Phytochemistry. 27: 1571–1574.
Mitchell, R.E. and Bieleski, R.L. 1977. Involvement of phaseolotoxin in halo blight of beans. Transport and conversion to functional toxin. Plant Physiol. 60: 723–729.
Templeton, M.D., Sullivan, R.E. and Shepherd, M.G. 1985. The inactivation of ornithine transcarbamoylase by N∂(-N′-sulpho-diaminophosphinyl)-L-ornithine. Biochem. J. 228: 347–352.
Ferguson, A.R. and Johnston, J.S. 1980. Phaseolotoxin: chlorosis. ornithine accumulation and inhibition of ornithine carbamoyltransferase in different plants. Physiological Ann. Pathol. 16: 269–275.
Hartman, C.L., Secor, G.A., Venette, J.R. and Albaugh, D.A. 1985, Response of bean calli to filtrate from Pseudamonas syringae pv. phaseolicola and correlation with whole plant disease reaction. Physiol. Mol. Plant Pathol. 28: 353–358.
Staskawicz, B.J. and Panopoulos, N.J. 1979. A rapid and sensitive microbiological assay for phaseolotoxin. Phytopathology 69: 663–666.
Staskawicz, B.J., Panopoulos, N.J. and Hoogenraad, N.J. 1980. Phylotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola. Basis for immunity to phaseolotoxin. J. Bacteriol. 142: 720–723.
Templeton, M.D., Sullivan, P.A. and Shepherd, M.G. 1986. Phaseolotoxin-insensitive L-ornithine transcarbamoylase from Pseudomonas syringae pv. phaseolicola. Physiol. Mol. Plant Pathol. 29: 393–403.
Mosqueda, G. Van den Broeck, G., Saucedo, O., Bailey, A., Alvarez-Morales, A. and Herrera-Estrella, L,. 1990. Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferas. Mol. Gen Genet. 222: 461–466.
Sanford, J.G. and Johnston, S.A. 1985. The concept of parasite-derived resistance-deriving resistance genes from the parasite's own genome. J. Theor. Biol. 113: 395–405.
Taylor, A.A. and Stewart, G.R. 1981. Tissue and subcellular localization of enzymes of arginine metabolism in Pisum sativum. Biochim. Biophys. Res. Comm. 101: 1281–1289.
Van den Broeck, G., Timko, M.P., Kaush, A.P., Cashmore, A.R., Van Montagu, M. and Herrera-Estrella, L. 1985. Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase. Nature 313: 358–363.
Patil, S.S., Tam, L.Q. and Sakai, W.S. 1972. Mode of action of the toxin from Pseudomonas phaseolicola. I. Toxin specificity, chlorosis and ornithine accumulation. Plant Physiol. 42: 803–807.
Smith, A.G. and Rubery, P.H. 1982. Investigation of the mechanism of action of a chlorosis-inducing toxin produced by Pseudomonas phaseolicola. Plant Physiol. 70: 932–938.
Turner, J.G. 1986. Effect of phaseolotoxin on the synthesis of arginine and protein. Plant Physiol. 80: 760–765.
Anzai, H., Yoneyama, K. and Yamaguchi, I. 1989. Transgenic tobacco resistant to a bacterial disease by the detoxification of a pathogenic toxin. Mol. Gen. Genet 219: 492–494.
Panopoulos, N.J. and Peet, R.C. 1985. The molecular genetics of plant pathogenic bacteria and their plasmid. Ann. Rev. Phytopathol 23: 381–419.
Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Teeri, T.H. 1988. The study of gene regulation and protein targeting in plant cells using gene fusion techniques. Ph. D. Thesis, University of Helsinki, Helsinki Finland.
Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G. and Fraley, R.T. 1985. A simple and general method for transferring genes into plants. Science 227: 1229–1231.
Ceriotti, G. 1973. Optimal conditions for ornithine carbamoyl transferase determination. A simple micromethod without deproteinization. Clin. Chim. A. 47: 97–105.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Cerovic, Z.G. and Plesnicar, P.M. 1984. An improved procedure for the isolation of intact chloroplasts of high photosynthetic capacity. Biochem. J. 223: 543–545.
Wharton, D.L. and McCarty, R.E. 1972. Experiments and Methods in Biochemistry, McMillan Publishing, NY.
Sequeira, L. Bacterial hypersensitivity. In: Nicotiana Procedures for Experimental Use. Durbin, R. D. (Ed.). Technical bulletin 1586, USDA.
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Fuente-Martínez, J., Mosqueda-Cano, G., Alvarez-Morales, A. et al. Expression of a Bacterial Phaseolotoxin–Resistant Ornithyl Transcarbamylase in Transgenic Tobacco Confers Resistance to Pseudomonas Syringae pv. Phaseolicola. Nat Biotechnol 10, 905–909 (1992). https://doi.org/10.1038/nbt0892-905
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DOI: https://doi.org/10.1038/nbt0892-905