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Field Resistance of Transgenic Russeet Burbank Potato to Effects of Infection by Potato Virus X and Potato Virus Y

Bio/Technology volume 8, pages 750–754 (1990)Cite this article

Abstract

Transgenic Russet Burbank potato plants expressing the coat protein genes of potato virus X (PVX) and potato virus Y (PVY) were transplanted into the field after propagation in tissue culture. Virus resistance, plant growth and tuber yield were determined. Our results show that expression of PVX and PVY CP genes confer a very high level of resistance to PVX and PVY infection in clone 303, one of the four different transgenic potato clones tested. After inoculation with PVX and PVY, tuber yield of control Russet Burbank plants decreased, while the yield of clone 303 was unaffected. Four different uninoculated transgenic clones had tuber yields as high as control Russet Burbank. These results confirm that resistance to PVX and PVY is effective in the field and can prevent yield losses due to dual infection by these viruses.

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References

  1. Beemster, A.B.R and deBokx, J.A. 1987. Survey of Properties and Symptoms. In: Viruses of Potatoes and Seed potato Production, p. 84–113. J. A. deBokx and J. P. H. Van der Want, (Eds). Wageningen, Netherlands.

    Google Scholar 

  2. de Bokx, J.A. 1986. Potato Virus Y. In: Compendium of Potato Diseases, p. 70–71 . W. J. Hooker, (Ed.) American Phytopathological Society.

    Google Scholar 

  3. Munro, J. 1986. Potato Virus X. In: Compendium of Potato Diseases, p. 72–74. W. J. Hooker, (Ed). American Phytopathological Society.

    Google Scholar 

  4. Powell-Abel, P., Nelson, R.S., De, B., Hoffmann, N., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.

    Article  Google Scholar 

  5. Tumer, N.E., O'Connell, K.M., Nelson, R.N., Sanders, P.R., Beachy, R.N., Fraley, R.T. and Shah, D.M. 1987. Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO J. 6: 1181–1188.

    Article  CAS  Google Scholar 

  6. Loesch-Fries, L.S., Merlo, D., Zinnen, T., Burhop, L., Hill, K., Krahn, K., Jarvis, N., Nelson, S. and Halk, E. 1987. Expression of alfalfa mosaic virus RNA4 in transgenic plants confers virus resistance. EMBO J. 6: 1845–1851.

    Article  CAS  Google Scholar 

  7. Van Dun, C.M.P., Bol, J.F. and van Vloten-Doting, L. 1987. Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159: 299–305.

    Article  CAS  Google Scholar 

  8. Cuozzo, M., O'Connell, K.M., Kaniewski, W., Fang, R.-X., Chua, N.-H. and Tumer, N.E. 1988. Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6: 549–557.

    CAS  Google Scholar 

  9. Hemenway, C., Fang, R.-X., Kaniewski, W.K., Chua, N.-H. and Tumer, N.E. 1988. Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO J. 7: 1273–1280.

    Article  CAS  Google Scholar 

  10. Van Dun, C.M.P., Overduin, B., van Vloten-Doting, L. and Bol, J.F. 1988. Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross protect against alfalfa mosaic virus infection. Virology 164: 383–389.

    Article  CAS  Google Scholar 

  11. Van Dun, C.M.P. and Bol, J.F. 1988. Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167: 649–652.

    Article  CAS  Google Scholar 

  12. Hemenway, C., Tumer, N.E., Powell, P.A. and Beachy, R.N. 1989. Genetic Engineering of plants for Viral Disease Resistance. In: Cell Culture and Somatic Cell Genetics, p. 405–423. J. Schell and I. Vasil (Eds.) Academic Press, Inc., San Diego, California.

    Google Scholar 

  13. Lawson, C., Kaniewski, W., Haley, L., Rozman, R., Newell, C., Sanders, P. and Tumer, N.E. 1990. Engineering resistance to Multiple virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Y in transgenic Russet Burbank potato. Bio/Technology 8: 127–134.

    CAS  PubMed  Google Scholar 

  14. Nelson, R.S., McCormick, S.M., Delannay, X., Dube, P., Layton, J., Anders, J., Kaniewska, M., Proksch, R.K., Horsch, R.B., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1988. Virus tolerance, plant growth and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.

    Google Scholar 

  15. Hoekema, A., Huisman, M.J., Molendijk, L., van den Elzen, P.J.M. and Cornelissen, B.J.C. 1989. The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technoiogy 7: 273–278.

    Google Scholar 

  16. Yang, I., Reddick, B. and Slack, S.A. 1983. Results of experiments on the purification of potato virus Y. Phytopathology 73: 794–797.

    Google Scholar 

  17. Shepard, J.F. and Shalla, T.A. 1972. Relative antigenic specificities of two PVX strains and their d-protein oligomers. Virology 47: 54–60.

    Article  CAS  Google Scholar 

  18. Stark, D.M. and Beachy, R.N. 1989. Protection against potyvirus infection in transgenic plants: Evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262.

    Google Scholar 

  19. Einot, I. and Gabriel, K.R. 1975. A study of the powers of several methods of multiple comparisons. J. of the Amer. Statistical Association 70: 351.

    Article  Google Scholar 

  20. Rochow, W.F., Ross, A.F. and Siegel, B.M. 1955. Comparison of local-lesion and electron microscope particle count methods for assay of potato virus X from plants doubly infected by potato viruses X and Y. Virology 1: 28–39.

    Article  CAS  Google Scholar 

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Author notes

  1. Nilgun E. Tumer: Corresponding author.

Authors and Affiliations

  1. Plant Sciences Department, Monsanto comapny, 700 Chesterfield Village Parkway, St. Louis, MO, 63188

    Wojciech Kaniewski, Cliff Lawson, Bernard Sammons, Lisa Haley, Jesse Hart, Xavier Delannay & Nilgun E. Tumer

Authors
  1. Wojciech Kaniewski
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  2. Cliff Lawson
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  3. Bernard Sammons
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  4. Lisa Haley
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  5. Jesse Hart
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  6. Xavier Delannay
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  7. Nilgun E. Tumer
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Kaniewski, W., Lawson, C., Sammons, B. et al. Field Resistance of Transgenic Russeet Burbank Potato to Effects of Infection by Potato Virus X and Potato Virus Y. Nat Biotechnol 8, 750–754 (1990). https://doi.org/10.1038/nbt0890-750

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