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Transgenic Plants of Brassica napus L.

Bio/Technology volume 5, pages 815–817 (1987)Cite this article

Abstract

Longitudinal stem sections of Brassica napus L. cv. Westar were co–cultivated with Agrobacterium tumefaciens A208–E, carrying the disarmed plasmid pTiT37–SE and the binary vector pMON809. The vector contains a mouse mutant dihydrofolate reductase (dhfr) coding sequences driven by the cauliflower mosaic virus 35S promoter. Inoculated explants produce transgenic shoots in selective media containing methotrexate (MTX) at a frequency of 10%. The transgenic plants are phenotypically normal, express the 35S–dhfr mRNA and are resistant to high concentrations of MTX. The mouse mutant dhfr gene is transmitted to and expressed in the seed progeny as a dominant Mendelian trait.

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Author notes

  1. Eng-Chong Pua

    Present address: Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Singapore, 0511, Republic of Singapore

  2. Nam-Hai Chua: To whom correspondence should be addressed.

Authors and Affiliations

  1. Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York, 10021-6399

    Eng-Chong Pua, Asha Mehra-Palta, Ferenc Nagy & Nam-Hai Chua

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  1. Eng-Chong Pua
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  3. Ferenc Nagy
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Pua, EC., Mehra-Palta, A., Nagy, F. et al. Transgenic Plants of Brassica napus L.. Nat Biotechnol 5, 815–817 (1987). https://doi.org/10.1038/nbt0887-815

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