Nat. Biotechnol. 10.1038/nbt.3267; corrected online 18 June 2015

In the version of this article initially published online, in Figure 3c, the label “unidentified b-type ions” should have been deleted, and in Figure 4, the symbols over the peptide sequences were misplaced. The errors have been corrected for the print, PDF and HTML versions of this article.