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How does eukaryotic gene prediction work?

Computational prediction of gene structure is crucial for interpreting genomic sequences. But how do the algorithms involved work and how accurate are they?

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Figure 1: Hidden Markov models form the basis for most gene-prediction algorithms.


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I wish to thank Jeltje van Baren and Tamara Doering for helpful comments and Laura Kyro for artwork. M.R.B. is supported in part by grants from the National Institutes of Health (HG002278, HG003700), the National Science Foundation (0501758) and Monsanto.

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Brent, M. How does eukaryotic gene prediction work?. Nat Biotechnol 25, 883–885 (2007).

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