Abstract
We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region with an inserted antigen. In vitro, such antibodies make B cells approximately 1000-fold more efficient at presenting antigen and stimulating specific T cells. In vivo, the antibodies turn B cells of the spleen into potent stimulators of T cells. This approach may be useful for the generation of new vaccines.
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Acknowledgements
We thank Hilde Omholt and Peter Hofgaard for excellent technical assistance, Ingunn B. Rasmussen and Janne K. Eidem for helpful discussions, and Joel Glover for reading the manuscript. The work was supported by grants from The Norwegian Research Council and The Norwegian Cancer Society.
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Lunde, E., Munthe, L., Vabø, A. et al. Antibodies engineered with IgD specificity efficiently deliver integrated T-cell epitopes for antigen presentation by B cells. Nat Biotechnol 17, 670–675 (1999). https://doi.org/10.1038/10883
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DOI: https://doi.org/10.1038/10883
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